Main Article Content

Dr. Thanneru Manohar
Dr. Swarna Deepak Kuragayala
Dr. Lone Dilip
Dr. Pendela Nithesh Nand
Me. Jerome.U. Tamayao


Hepatoprotective Activity, Aqueous Leaf Extract, Pako (Diplazium esculentum), Chloroform, Induced Albino Rats.


Background: In this study, we wanted to determine the hepato-protective property of pako leaves extract (Diplazium esculentum) in Swiss albino rats and compare the effect of different rates of fiddle fern (Diplazium esculentum).

Methods: The in-vivo hepatoprotective effect of the aqueous extract of Diplazium esculentum (Retz.) Sw. against chloroform induced hepatotoxicity in albino rats was studied. The test rats were brought from PITAHC-DOH animal facility, the plant extraction was carried out at ROCO buildin, Cagayan State University (CSU) Carig Campus, College of Arts and Science, Carig Sur, Tuguegarao city, and the test was conducted at the PITAHC-DOH animal facility, after obtaining clearance from Institutional Ethics Committee.

Results: In the mean total protein, there was no significant mean difference between the treatments with the increase in dose rate of Fiddle fern extract given to the animals (P = 0.338), Significant differences were observed for the SGPT between the control group (T2) and the group treated with 20 g/kg extract (P = 0.024). There were no significant differences detected for the (T1) negative control group with T4-extract with 40 g/kg and T2-treated with essential.

Conclusion: The 40 g/kg of the fiddlehead fern extract prevents liver damage comparably similar to those with essential and the normal control group. To conclude, the death was prevented with administration of the extract 20 and 40 g/kg.

Abstract 51 | PDF Downloads 24


1. Alessandro C. Alcohol induced fatty liver and inflammation: where do Kupffer cells act? J Hepatol 2000;32(6):1026-30.
2. Mageswari B, Latha P, Rukkumani R. Effect of Phyllanthus niruri on alcohol and poly unsaturated fatty acid induced oxidative stress in liver. Int J Pharm Pharm Sci 2010;2(4):58-62.
3. Kim SH, Cheon HJ, Yun N, Oh ST, Shin E, Shim KS, Lee SM. Protective effect of a mixture of Aloe vera and Silybum marianum against carbon tetrachloride–induced acute hepatotoxicity and liver fibrosis. J Pharmacol Sci 2009;109(1):119-27.
4. Kulandairaj, D, John DB. Antibacterial and antifungal activity of secondary metabolities from some medicinal and other common plant species. J Eco Taxon Bot 2000;24:21.
5. Yumkham SD, Singh PK. Less known ferns and fern-allies of Manipur with ethnobotanic uses. Indian Journal of Traditional Knowledge 2011;10(2):287-91.
6. Sen A, Ghosh PD. A note on the ethnobotani- cal studies of some pteridophytes in Assam. Indian Journal of Traditional Knowledge 2011;10(2):292-5.
7. Ujowundu CO, Igwe CU, Eremor VA, Nwaogwu LA, Okafor OE. Nutritive and anti-nutritive properties of Boerhavia diffusa and Commelina nucliflora leaves. Pak J Nutr 2008;7(1):90-2.
8. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin Phenol reagents. J Biol Chem 1951;193:265-75.
9. Imray J, Kennedy BR, Kilpatrick SJ. Effect of brief administration of chloroform on the liver function. Anesthesia 2007;19(1):33-9.