FORMULATION AND CHARACTERIZATION OF ALKANNA TINCTORIA LOADED EMULGEL AND ITS IN VITRO AND IN VIVO EVALUATION AS A COSMECEUTICAL PRODUCT

Main Article Content

Muhammad Shahzad Khan
Mudassar Mazher
Qazi Adnan
Zafar Iqbal
Umer Shaukat
Hosh Muhammad Lashari
Abdul Quddoos
Mehran Khan
Shahzada Khurram
Umair Khurshid
Naheed Akhtar
Fahad Mehmood Khan
Marwah Rehman

Keywords

Alkannatinctoria, DPPH, Elastometer, Total phenolic contents, total flavonoids contents, skin pores

Abstract

Background: Herbs and plants with enormous levels of phenolic and flavonoid constituents are commonly utilized as antioxidant agents to reduce the impact of UV radiation on the skin and play a significant role in skin treatments.


Aims: Alkanna tinctoria extract's potential cosmetic benefits including reduction in melanin, erythema, sebum, anti-aging and sun protection effectswere investigated in this study, which also aimed to create a stable emulgel that contained 4% Alkanna tinctoria extract.


Objective: Alkanna tinctoria extract was examined and quantified for its phenol and flavonoid content in order to ascertain its cosmetic effects on human skin.


Methods: Alkanna tinctoria (AT) loaded o/w emulsion were combined with gel containing carbopol-940 as gelling agent to create the emulgel formulation. Same procedure and formulation was adopted for control emulgel without extract. For the duration of a 12-week storage period, an in vitro investigation was conducted to assess how color, liquefaction, pH and hardness changed. 13 healthy human volunteers' cheeks were treated with emulgel in order to assess its cosmetic benefits in comparison to control formulations. Utilizing the Mexameter, Elastometer, Sebumeter and Corneometer, and over the duration of the 12-week trial, facial parameters such as skin pigmentation, moisture content, redness, sebum, and skin elasticity were assessed.


Results: Alkanna tinctoria extract found to have 48.04 ± 0.0033 mg GAE/g of total phenolics, and flavonoids were 8.64 ± 0.0046 mg QE/g. Alkanna tinctoria emulgel significantly efficient at minimizing the sebum, small pores andlarge pores (p ≤ 0.05) while efficient to maximize skin’s elasticity and hydration as compared to control emulgel. Antioxidant activity by DPPH activity was 94.23±0.028.


Conclusion: The flavonoid and phenolic concentration of Alkanna tinctoria extract makes it a potent antioxidant that works to protect skin from photodamage and enhance skin conditions.

Abstract 155 | pdf Downloads 85

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