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Hafiza Ayesha Andleeb
Muhammad Usama Tariq
Faiza Javaid
Wajiha Yousuf
Sadaf Ali Jaffri
Ayesha Masood
Amina Mahmood
Nureen Zahra


Plant extracts Citrullus colocynthis Antimicrobial activity Antioxidant activity n-Hexane Methanol Acetone.


Citrullus colocynthis leaves, fruits, stem, and seeds have been found to offer high potential for producing plant protection products with a wide range of biological activities. As a result, in this investigation, we describe the possible antibacterial and anticancer characteristics of C. colocynthis seeds extracted with different polarity solvents, such as methanol (ME), hexane (H.E.), and chloroform (A.E.). C. colocynthis seed, fruit, stem, and leaf extracts were tested for antibacterial activity against gram-positive and gram-negative bacteria and fungus. All  extracts exhibited notable antibacterial activity with  minimum inhibitory concentrations (MICs) ranging from 70% against Lactobacillus acidophilus (ATCC4356) and Streptococcus equinus (ATCC 9812); At the same time, they were found to have moderate activity (MIC 80%) against Enterococcus faecalis (ATCC29212) and Enterobacter sakazakii (ATCC29544) strains and had the highest antibacterial activity against Enterobacteriaceae (ATCC25922, Bacillus Alcalophilus (ATCC27647) ) and Listeria monocytogenes (ATCC 13932) with an MIC value of 100%, which is equal to the standard dose of the positive control drug tobramycin in this study. While for the antifungal drugs hexane and methanol , all extracts had the lowest MIC (0%) against S. Cervisiae (ATCC 9763) and moderate activity (MIC 60%) against Rhizopus spp (ATCC 52813). The extract was spectrophotometrically investigated using radical scavenging with 1, 1-diphenyl-2-picryl hydrazyl. Citrullus colocynthis extracts in methanol, acetone, and n-hexane inhibited the most. Because of the seed extract's high antioxidant ability, the 1, 1-diphenyl-2-picryl hydrazyl technique yielded 0.15% and hydrogen peroxide yielded 10_50 g mL-1, respectively. As a result, the primary goal of this thorough study is to offer an overview of the data on the beneficial and detrimental effects of C. colocynthis ingestion on human health. This narrative piece will continue to discuss studies on the potential of this intriguing natural fruit and its bioactive chemicals in human medicine as nutraceuticals and superior therapeutic medications. To the best of our knowledge, all of the plant components found in this investigation were extracted for the first time from C. colocynthis.

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