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Wound, excision, Hydrocotyle sibthorioides, extract, toxicity
The objective of the present investigation was to evaluate the wound healing action of Hydrocotyle sibthorpioides leaf extract in rats. The extraction abilities of different solvents for recovering extractable components from leaves followed the order: ethanol >water>pet ether. The acute toxicity study suggests a LD50 of more than 2000 mg/Kg for both the ethanolic and aqueous extracts of the plant. The ethanolic and aqueous extracts of Hydrocotyle sibthorpioides leaves were evaluated for the in vivo wound healing effect by the excision model. The topical application of 5 % w/w of the Hydrocotyle sibthorpioides resulted in an enhanced and statistically significant (p < 0.05) wound healing activity in vivo when using the ethanolic extract. The wound healing by the aqueous extract was not significant in comparison to the control group. The ointment containing plant extract (ethanolic) exhibited 98.56 ± 3.61 % contraction of wound on the 21st day whereas only 63.99 ± 6.33 % contraction of wound was found in the control animals. The results revealed by aqueous extract were also significant as it could reduce the wound area by 88.89 ± 6.33 % on the 21st day.
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