Main Article Content
Convolvulaceae, Constipation, Laxative, Animal Model, Medicinal plant, Convolvulus spinosus
Convolvulus spinosus Burm.f. (C. spinosus) is a woody shrub and is traditionally used in Pakistan for its laxative virtue in indigenous culture. Despite the usage of C. spinosus by traditional healers and local communities for the management of constipation, it lacks scientific evidence for its claimed traditional use. The aim of the study is to evaluate the laxative effect of 70% ethanolic extract of C. spinosus in mice. The laxative potential of 70% ethanolic extract of C. spinosus (100/200 and 400 mg/kg P.O.) was evaluated and analyzed for the mean number of fecal pellets, percentage of fecal pellet water content (laxative test), and gastrointestinal transit ratio by charcoal meal movement in the small intestine in the Loperamide-induced constipated mice model, and intestinal fluid accumulation in normal mice. The phytochemical screening and ATR-FTIR analysis of C. spinosus 70% ethanolic extract were performed. The laxative test demonstrated that C. spinosus 70% ethanolic extract indicated a significant increase in both the number of fecal pellets at 200 mg/kg (p<.05) and 400 mg/kg (p =.0001), the percent fecal water content at 100 mg/kg (p<.0001), 200 mg/kg (p<.0001) and 400 mg/kg (p<.0001) in loperamide-induced constipated mice model. The gastrointestinal motility test of C. spinosus 70% ethanolic extract showed significant accelerated propulsion of charcoal meal in loperamide-induced constipated mice and produced a significant increase in Gastrointestinal (GI) transit ratio at 100 mg/kg (p<.05), 200 mg/kg (p<.0001) and 400 mg/kg (p<.0001). The findings of the Gastrointestinal secretion test presented that C. spinosus extract produced significantly higher fluid accumulation at 200 mg/kg (p <0.05) and 400 mg/kg (p<0.0001). The phytochemical screening of C. spinosus extract showed the presence of tannins, alkaloids, terpenoids, and flavonoids. This study provides scientific evidence for the traditional use of C. spinosus for the treatment of constipation and highlights the need for the identification of biologically active compound(s).
2. Wlodarczyk J, Wasniewska A, Fichna J, Dziki A, Dziki L, Wlodarczyk M. Current overview on clinical management of chronic constipation. J Clin Med 2021;10:1738.
3. Jani B, Marsicano E. Constipation: evaluation and management. Mo Med 2018;115(3):236.
4. Serra J, Mascort-Roca J, Marzo-Castillejo M, Aros SD, Santos JF, Rubio ERD, Manrique FM. Clinical practice guidelines for the management of constipation in adults. Gastro Hep 2017;40:132-141.
5. Soheilipour M, Goudarzinejad E, Tabesh E. Efficacy of non-pharmacological treatment for adult patients with chronic constipation. Int J Phys Patho Phys Pharm 2022;14:247.
6. Portalatin M, Winstead N. Medical management of constipation. Clini Col Rect Surg 2012;25:012-019.
7. Abdolshahi A, Atashi NS, Majd HM, Salehi B, Kobarfard F, Ayatollahi SA, Sharifi-Rad J. Antibacterial activity of some Lamiaceae species against Staphylococcus aureus in yoghurt-based drink (Doogh). Cell Mol Biol 2018;64:71-77.
8. Austin DF. Convolvulaceae (Morning Glory Family). Available online:
http://ag.arizona.edu/herbarium/personnel/daustin/convolv.html, retrieved on August 20, 2020.
9. Salehi B, Krochmal‐Marczak B, Skiba D, Patra JK, Das SK, Das G, Martorell M. Convolvulus plant—A comprehensive review from phytochemical composition to pharmacy. Phyt Res 2020;34:315-328.
10. Al-Snafi AE. The chemical constituents and pharmacological effects of Convolvulus arvensis and Convolvulus scammonia-A review J Pharm, 2016;6:64-75.
11. Hameed S. Flora of Pakistan: Convolvulus spinosus Burm.f.. Available online:
http://www.efloras.org/florataxon.aspx?flora_id=5&taxon_id=230001339, retrieved on April 20, 2020.
12. Goodman SM, Ghafoor A. The Ethnobotany of Southern Balochistan, Pakistan, with Particular Reference to Medicinal Plants. FIELDIANA: BOT. Published by Field Museum of Natural History1992:37.
13. World Flora Online. Version.[December]. Available online
http://www.worldfloraonline.org/search?query=Convolvulus+spinosus data retrieved on April 21, 2023
14. Abubakar AR, Haque M. Preparation of medicinal plants: Basic extraction and fractionation procedures for experimental purposes. J Pharm Bioall Sci 2020; 12:1.
15. Jones‐Bolin, S., 2012. Guidelines for the care and use of laboratory animals in biomedical research. Curr Protoc Pharm. 2012;59: A-4B.
16. Anonymous. OECD guidelines for the testing of chemicals: acute oral toxicity: up and down procedures. Accessed Online:
17. Adeniyi OS, Edache M, Abi I, Ediale R. Ameliorative effects of virgin coconut oil in loperamide induced constipation in rats. J Biomed Res Clin Pract 2020;3:309-315.
18. Li C, Nie SP, Zhu KX, Xiong T, Li C, Gong J, Xie MY. Effect of Lactobacillus plantarum NCU116 on loperamide-induced constipation in mice. Int J foo Sci Nutri 2015; 66:533-538.
19. Tessema MY, Wubneh ZB, Asrie AB. Laxative activities of 80% methanolic extract of the leaves of grewia ferruginea hochst ex a rich in mice. J Evi-Ba. Int Med 2020; 25, 2515690X20926922.
20. Choi JS, Kim JW, Kim KY, Lee JK, Sohn JH, Ku SK. Synergistic effect of fermented rice extracts on the probiotic and laxative properties of yoghurt in rats with loperamide-induced constipation. Evi-Bas. Complement. Alt. Med., https://doi.org/10.1155/2014/878503.
21. Robert A, Nezamis JE, Lancaster C, Hanchar AJ, Klepper MS. Enteropooling assay: a test for diarrhea produced by prostaglandins. Prostaglandin 1976;11:809-828.
22. Auwal MS, Saka S, Mairiga IA, Sanda KA, Shuaibu A, Ibrahim A. Preliminary phytochemical and elemental analysis of aqueous and fractionated pod extracts of Acacia nilotica (Thorn mimosa). In Vet Rese An Int Quat J 2014;5:95.
23. Odeja O, Gwuche CE, Elemike EE, Obi G. Phytochemical screening, antioxidant and antimicrobial activities of Acalypha Ciliate Plant. Clini Phytosci 2017;2:1-6.
24. Kongdang P, Pruksakorn D, Koonrungsesomboon N. Preclinical experimental models for assessing laxative activities of substances/products under investigation: a scoping review of the literature. Am J Transl Res 2022;14:698.
25. Meite S, Bahi C, Yeo D, Datte JY, Djaman JA, Nguessan DJ. Laxative activities of Mareya micrantha (Benth.) Müll. Arg (Euphorbiaceae) leaf aqueous extract in rats. BMC Comp. Alt Med 2010;10:1-6.
26. Seo JY, Kim SS, Kim HJ, Liu KH, Lee HY, Kim JS. Laxative effect of peanut sprout extract. Nutr Res Pract 2013;7:262-266.
27. Wald A. Is chronic use of stimulant laxatives harmful to the colon?. J Clin Gastro 2003;36:386-389.
28. Krogh K, Chiarioni G, Whitehead W. Management of chronic constipation in adults. United. Euro Gast.J 2017;5:465-472.
29. Camilleri M, Ford AC, Mawe GM, Dinning PG, Rao SS, Chey WD, Chang L. Chronic constipation. Nat Rev Dise Prim 2017;3:1-19.
30. Ayele AG, Mulugeta B, Wondmkun YT. Evaluations of the in vivo laxative effects of aqueous root extracts of Euclea racemosa L. in mice. Metab Ope 2023;17:100222.
31. Sayuk GS, Waldman SA, Brenner DM. Mechanisms of action of current pharmacologic options for the treatment of chronic idiopathic constipation and irritable bowel syndrome with constipation. Am J Gast 2022;117:S6-S13.
32. Kim JE, Choi YJ, Lee SJ, Gong JE, Jin YJ, Park SH, Hwang DY. Laxative effects of phlorotannins derived from Ecklonia cava on loperamide-induced constipation in SD rats. Mol 2021;26:7209.
33. Nyamai DW, Arika W, Ogola PE, Njagi ENM, Ngugi MP. Medicinally important phytochemicals: an untapped research avenue. J Pharmacogn Phytochem 2016;4:35-49.
34. Vivekanandan L, Mandere RG, Thangavel S. Evaluation of the Laxative Activity of Saponin Enriched Hydroethanolic Pericarp Extract of Sapindus emarginatus in Animal Models. Curr Bioact Comp 2021;17:40-46.
35. Zhan W, Yang X, Lu G, Deng Y, Yang L. A rapid quality grade discrimination method for Gastrodia elata powderusing ATR-FTIR and chemometrics. Spectrochim Act Pt A Mol Biomol Spectro 2022;264:120189.
36. Geyik GA, Cengiz BP, Tugrul F, Elmas SNK, Yilmaz I, Arslan FN. A rapid diagnostic approach for gastric and colon cancers via Fourier transform mid–infrared spectroscopy coupled with chemometrics from paraffin–embedded tissues. Spectrochimi. Act Pt A Mol Biomol Spect 2022;267:120619.
37. Coury C, Dillner AM. ATR-FTIR characterization of organic functional groups and inorganic ions in ambient aerosols at a rural site. Atm Env 2009;43:940-948.
38. Pakkirisamy M, Kalakandan SK, Ravichandran K. Phytochemical screening, GC-MS, FTIR analysis of methanolic extract of Curcuma caesia Roxb (Black Turmeric). Pharmacogn J 2017;9(6).
39. Ashokkumar R, Ramaswamy M. Phytochemical screening by FTIR spectroscopic analysis of leaf extracts of selected Indian medicinal plants. Int J Curr Micrbio Appl Sci 2014;3:395-406.
40. Singh PK, Singh J, Medhi T, Kumar A. Phytochemical Screening, Quantification, FT-IR Analysis, and In Silico Characterization of Potential Bio-active Compounds Identified in HR-LC/MS Analysis of the Polyherbal Formulation from Northeast India. ACS Omeg 2022;7: 33067-33078.