EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF AMALGAMATION OF KUTKUTI (TRIDAX PROCUMBENS) AND GINGER JUICE AGAINST PARACETAMOL INDUCED HEPATOTOXICITY IN RATS.

Main Article Content

Sucheta S. Pawar
Atish B. Velhal
Vitthal J. Chaware
Vivekkumar K. Redasani

Keywords

Amalgamation, of, tridax, procumbens, and ginger, juice, heptoprotection, paracetamol, rats, SGOT, SGPT, ALP

Abstract

The hepatoprotective activity of amalgamation of tridax procumbens and ginger juice was evaluated against paracetamol induced hepatotoxicity in rats. The albino wistar rats (n = 30) were randomly divided into 6 groups (n = 5). Group I (Normal control) did not receive any treatment. Group II (Toxic control) animals were administered with paracetamol (2.75g/kg p.o.) on 6th and 7th day. Group III rats pretreated with silymarin at a dose of 50 mg/kg b. w. /day for 7 days and were induced with Paracetamol at dose 2.75g/kg p.o. on 6th and 7th day. Group IV rats pretreated with ethanolic extract of tridax procumbens at dose 200mg/kg for 7 days and were induced with paracetamol at dose 2.75g/kg p.o. on 6th and 7th day . Group V rats pretreated with ginger juice at dose 2ml/kg for 7 days and were induced with paracetamol at dose 2.75g/kg p.o. on 6th and 7th day. Group VI pretreated with ethanolic extract of tridax procumbens and ginger juice at 200 mg/kg and 2ml/kg dose b. w. /day for 7days and were induced with paracetamol at dose 2.75g/kg p.o. on 6th and 7th day. Liver function tests, triglyceride, MDA, glutathione and electrolyte profile were estimated using standard kits. Livers were quickly removed and fixed in 10% formalin and subjected to histopathological studies. In conclusion, the amalgamation of tridax procumbens and ginger juice can enhance antioxidant activity and ameliorate the paracetamol-induced hepatotoxicity. The results of this study strongly indicate that Amalgamation of tridax procumbens and ginger juice has got a potent hepatoprotective action against paracetamol induced hepatic damage in rats.

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References

1. Zimmerman HJ, Ishak KG, MacSween R, Anthony PP, Scheuer PJ, Burt AD, et al. Edinburgh: Churchill Livingstone; 1994. Hepatic injury due to drugs and toxins, in Pathology of Liver; pp. 563–634. [Google Scholar]
2. Boyd EH, Bereczky GM. Liver necrosis from paracetamol. Br J Pharmacol. 1966;26:606–14. [PMC free article] [PubMed] [Google Scholar]
3. Dahlin D, Miwa G, Lu A, Nelson S. N-acetyl-p-benzoquinone imine: A cytochrome P-450- mediated oxidation product of acetaminophen. Proc Natl Acad Sci. 1984;8:1327–31. [PMC free article] [PubMed] [Google Scholar]
4. Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glulathione reductase and glutathione-S-transferase activities in rat lung and liver. Biochim Biophys Acta. 1979;582:67–78. [PubMed] [Google Scholar]
5. Gupta AK, Chitme H, Dass SK, Misra N. Hepatoprotective activity of Rauwolfia serpentina rhizome in paracetamol intoxicated rats. J Pharmacol Toxicol. 2006;1:82–8. [Google Scholar]
6. Subramanian S, Khan HBH, Elumalai N, Lakshmi SYS. Hepatoprotective effect of ethanolic extract of whole plant of Andrographis paniculata against CCL4-induced hepatotoxicity in rats. Coparative clinical pathology.2015; 24(5):1-7
7. Chattopadhyay RR. Possible mechanism of hepatoprotective activity of Azadirachta indica leaf extract: Part II. J Ethnopharmacol. 2003;89:217–9. [PubMed] [Google Scholar]
8. Wolf PL. Biochemical diagnosis of liver disease. Indian J Clin Biochem. 1999;14:59–64. [PMC free article] [PubMed] [Google Scholar]
9. TortoraG.J,GrabowskiS.R.PrinciplesofAnatomyandPhysiology.8the d.Newyork:Harper Collins College;1996.p.824.
10. Junqueira LC, Carneiro J. Basic Histology Text and Atlas.10th ed .New York :Lnage medical books,McGraw Hill company;2003.p.332-343.


11. Ward F.M, Daly M.J; Hepatic disease.InWalkarR,Edward C, editors;clinical pharmacy and therapeutics .Churchi lLivingston,New York,1999;195-212.
12. Jude CI, Catherine CI and Ngozi MI. "Chemical profile of Tridax procumbens Linn. Pakistan Journalof Nutrition". vol.8,no.5,(2009),pp.548-550
13. Saraf S, Dixit VK. Hepatoprotective activity of Tridax procumbens - Part II. Fitoterapia 1991;62:534-6.
14. Ravikumar V, Kanchi Subramanian Shivashangari, Devaki T, Eff ect of Tridax procumbens on liver antioxidant defense system during lipopolysaccharide-induced hepatitis in Dgalactosamine sensitised rats. Molecular and Cellular Biochemistry, 2005;269: 131-136.
15. Hemant P. Evaluation of hypoglycemic and anti-hyperglycemic potential of Tridax procumbens.2009; [Google Scholar]
16. Ali M, Ravinder E, Ramachandran R. A new flavonoid from the aerial parts of Tridax procumbens. Fitoterapia 2001;72:313-5.
17. Saraf S, Pathak AK, Dixit VK. Hair growth promoting activity of Tridax procumbens. Fitoterapia 1991;62:495-8.
18. Ahmed RS, Sharma SB. Biological studies on combined effects of garlic (Allium sativum Linn.) and ginger (Zingiber officinale Roscoe.) in albino rats. J Exp Biol 1997; 35: 841-3.
19. Kikuzaki H, Nakatani N. Antioxidant effects of ginger constituents. J Food Sci 1993; 58:1407- 10.
20. Nicoll R, Henein MY. Ginger (Zingiber officinale Roscoe): a hot remedy for cardiovascular disease. Int J Cardiol 2009; 131(3): 408-9.
21. Doumas BT. Candidate reference methods for determination of total bilirubin in serum: development and validation. Clin Chem. 1985;31:1779e1783.
22. Sing S, Yuvaraj S, Malini T. Experimental diabetes has adverse effects on protein metabolism of rats. Discov Mol Cell Evol Biol. 2005;281:1061e1069.
23. Henry RJ, Chiamori M, Golub OJ, et al. Revised spectrophotometric methods for the determination of glutamate oxaloacetic transaminase, glutamic pyruvate transaminase and lactic acid dehydrogenase. Am J Clin Pathol. 1960;34:381e398.
24. Horecker BL. Alkaline phosphatase. In: Methods of Enzymology. New York: Academic Press; 1966:639e642.
25. Gutteridge J, Wilkins S. Copper-dependent hydroxyl radical damage to ascorbic acid. FEBS Letters. 1982;137:327–330. [PubMed] [Google Scholar]
26. Chaudhari B. P. et.al., Hepatoprotective activity of Hydroalcoholic extract of Momordica charantia Linn. leaves against Carbon tetra chloride induced Hepatopathy in Rats. 2009; 355-358
27. Ikewuchi C.C. et.al., Moderation of doxorubicin-induced nephrotoxicity in Wistar rats by aqueous leaf-extracts of Chromolaena odorata and Tridax procumbens. Porto Biomed J. 2021;6:e129. [PMC free article] [PubMed] [Google Scholar]