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Nimesh Kumar Dubey
Ritesh Jain
Neeraj Sharma
Dharmendra Singh Rajput


Anti-fungal, Graphene, Nano-precipitation, Nano-ointment, Usnic acid


Objective: The primary objective of this work is to examine the antifungal properties of usnic acid, a secondary metabolite found in lichens, when combined with graphene as a carrier.

Methods: The formulation of the nano-ointment combining graphene and usnic acid was achieved using the nano-precipitation technique using sonication. The in-vitro antifungal activity was conducted in order to validate the antifungal efficacy of the nano-ointment formulation.

Result: In the context of treating fungal infections, it is more appropriate to utilize topical medication administration. Therefore, the ointment was formulated by including the nano-combination using geometric mixing. A four-day in-vitro investigation was conducted to investigate the anti-fungal properties. The study revealed that usnic acid in its regular form, as well as graphene in its nano form, had anti-fungal properties, as evidenced by the observed zone of inhibition of 3mm and 5mm, respectively.

Conclusion: In conclusion, the findings of this study demonstrate that the nano-ointment formulation of usnic acid with graphene exhibited superior anti-fungal action compared to the conventional formulation.

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