"EXPLORING THE MECHANISMS OF HYPOTENSIVE REFLEXES ELICITED BY OZONE THERAPY: AN EXPERIMENTAL STUDY"

Main Article Content

Kupriyanov Sergey Vladilenovich
Mohammad Alhasan
Uchterova Nadezhda Dimitrievna

Keywords

vascular reflexogenic zone, vertebral artery, cardiorespiratory system, ozone, ozonetherapy

Abstract

We examined the effects of ozonated saline infusion into the hemodynamically isolated segment of vertebral arteries on cardiorespiratory reflexes in anesthetized cats. This intervention elicited concomitant reflex responses, such as systemic hypotension and respiratory depression. These cardiorespiratory inhibitory effects were specific to the initial perfusion with ozonated solutions.


Subsequent infusions of ozonated saline into the same isolated segment of vertebral arteries induced variable reflex responses of the respiratory and cardiovascular systems. These responses were either bidirectional or biphasic, with a predominance of a hypertensive response. These modifications were related to the nonspecific adaptive effects of ozone exposure.


Under the conditions of Novocain blockade of vertebral artery chemoreceptors, all the reflex responses were abolished. This confirmed their reflexogenic origin. Therefore, the effects of ozone exposure, similar to any other pharmacological agent used for therapeutic purposes, depend on its concentration in the organism. Ozone exposure exhibits both a local humoral effect, extensively documented in the literature, and general reflex effects that are demonstrated in this study. These reflex effects are mediated by vascular reflexogenic zones and their respective CNS centers.

Abstract 234 | PDF Downloads 86

References

1- Alekhina S.P. et al. Ozone therapy. - N.Novgorod: Vector-TiS, 1996. - 16 p.
2- Guyton A. Physiology of circulation: Cardiac output and its regulation. - M.: Medicine, 1969. - 472 p.
3- Kaminsky L.S. Medical and demographic statistics. - M.: Statistics, 1974. - 351 p.
4- Kupriyanov S.V. The state of systemic arterial pressure in cats during stimulation of chemoreceptors of the reflexogenic zone of vertebral arteries // Aviakosm. and ecol. medicine. - 1996. - V. 30, No. 1. - P. 60-62.
5- Kupriyanov S.V. Chemoreflexes of the zone of vertebral arteries and carotid sinus in acidosis and alkalosis in acute experiment and clinic // Kazan medical journal. - 2007. - V. 88, No. 1. - P. 20-23.
6- Kupriyanov S.V. Functional characteristics of the interoceptive zone of vertebral arteries // Morphological reports. - 2007. - No. 3-4. - P. 39-43.
7- Kupriyanov S.V., Agadzhanyan N.A. Reflexogenic zone of vertebral arteries. - Cheboksary: Publishing house of ChSU, 2005. - 136 p.
8- Kupriyanov S.V., Gavrishkina N.V. Chemoreceptor activity of the zone of vertebral arteries // Ross physiol journal named after I.M.Sechenov. - 2004. - V. 90, - No. 8. - P. 485-488.
9- Kupriyanov S.V., Kupriyanov V.S., Drandrov G.L., Vodyanov N.G. The role of reflexogenic zones of vertebral and carotid arteries in the formation of cardiovascular respiratory functional system // Bulletin of restorative medicine. - 2008. - No. 1 (23). - P. 78-85.
10- Lisitsyn D.M. et al . Kinetic parameters of oxidation of individual fatty acids by ozone // Bull exp biol and med. - 2004. - V. 138, No. 11. - P. 517-519.
11- Maslennikov O.V., Kontorschikova K.N. Practical ozone therapy. - N.Novgorod: Vector TiS , 2003. - 52 p.
12- Muratov I.D. Use of ozone for local treatment of purulent-inflammatory processes // Children's surgery. - 2005. - No. 1. - P. 50-53.
13- Mukhina I.V., Zhemarina N.V., Evdokimova O.S., Mironov A.A. Functional state of the rat brain during parenteral administration of ozonated saline solution // Materials XX Congress physiol. society named after I.P.Pavlov. - M., 2007. - P. 345.
14- Pavlova O.E. Influence of ozone on rheological properties of blood : Author's abstract dis cand biol sciences. - M., 1998. - 17 p.
15- Sizova E.N. et al . Influence of ozone on contractile activity and chemoreactivity of longitudinal musculature of the horn of the uterus of non-pregnant rats // Ross physiol journal named after I.M.Sechenov. - 2003. - V. 89, - No. 4. - P. 427-435.
16- Chandan K. Sen, Osmo Hänninen Glutathione homeostasis: cardiac physiological protection against oxygen toxicity caused by physical exercise // Physiol journal named after I.M.Sechenov. - 1995. - V. - 81, - No. 11. - P. 143-150.
17- Bocci V. Ozone as a bioregulator Pharmacology and toxicology of ozonetherapy today // J Biol Regulators and Homeostatic Agents. - 1997. - V. 10, - No 2/3. - P. 31-53.
18- Dotan Y et al Ibid - 2004. - No 43. - P 1-28.
19- Dvorak V. Ozone Use of ozone in industry and medicine // New in dentistry. - 2005. -V. 128, - No 4. - P. 84-88.
20- Minuz Pietro, Fava Cristiano, Cominacini Luciano. Oxidative stress, antioxidants and vascular damage // Brit J Clin Pharmacol. - 2006. - V. 61, - No 6. - P. 774-777.
21- Procaccini E.M., De Martino G., Picone G., D'Alicandro G., Monfrecola G.G. Effects of hyperbaric oxygen on cutaneous microflow // Ital Dermatol e Venereol. - 2005. - V. 140, - No 6. - P. 663-667.
22- Koller E.A. et al. Studies on the role of the lung deflation reflex // Respir Physiol. - 1970.
23- Nakao A., Sugimoto R., Billiar T.R., McCurry K.R. Therapeutic antioxidant medical gas // J Clin Biochem Nutr. - 2009. - No 44. - P. 1-13.
24- Anand I.S., Chandrashekhar Y., Rao S.K., Malhotra R.M., Ferrari R., Chandana J., Ramesh B., Shetty K.J., Boparai M.S. Body fluid compartments, renal blood flow, and hormones at 6, 000 m in normal subjects // J Appl Physiol. - 1993. - No 74. - P. 1234-1239. CAS PubMed Web of Science® Google Scholar.
25- Heindl S., Lehnert M., Criée C.-P., Hasenfuss G., Andreas S. Marked sympathetic activation in patients with chronic respiratory failure // Am J Crit Care Med. - 2001. – No 164, - P. 597-601. CAS PubMed Web of Science® Google Scholar.
26- Marshall J.M. Peripheral chemoreceptors and cardiovascular regulation // Physiol Rev. - 1994. - No 74. - P. 543-594. CAS PubMed Web of Science® Google Scholar.
27- Daly M., De B., Jones J.F. Respiratory modulation of carotid and aortic body reflex left ventricular inotropic responses in the cat // J Physiol. - 1998. - No 509. - P. 895-907. PubMed Web of Science® Google Scholar.
28- Greenwood P.V., Hainsworth R., Karim F., Morrison G.W., Sofola O.A. The effect of stimulation of carotid chemoreceptors on the inotropic state of the left ventricle // J Physiol. - 1976. - No 266. - P. 47P-48. PubMed Google Scholar.
29- Hainsworth R. Reflexes from the heart // Physiol Rev. - 1991. - No 71. - P. 617-658. CAS PubMed Web of Science® Google Scholar.
30- Narkiewicz K., Kato M., Pesek C.A., Somers V.K. Human obesity is characterized by a selective potentiation of central chemoreflex sensitivity // Hypertension. - 1999. - No 33. - P. 1153-1158. CAS PubMed Web of Science® Google Scholar.
31- Sevre K., Bendz B., Rostrup M. Reduced baroreceptor reflex sensitivity and increased blood pressure variability at 2400 m simulated cabin altitude // Aviat Space Environ Med. - 2002. - No 73. - P. 632-634. PubMed Web of Science® Google Scholar.
32- Dutta S., Zimmer M., Sies H., Carlson R.W., Cullen W.J., Thayer J.W. Comparative toxicology of ozone and t-butyl hydroperoxide on isolated rat lung // Free Radical Res Commun. - 1989. - V. 8, - No 1. - P. 27-35.
33- Fletcher G.F., Balady G.F., Froelicher V.F., Hartley L.H., Haskell W.L., Pollock M.L. Exercise standards. A statement for healthcare professionals from the American Heart Association. Writing Group Special Report // Circulation. - 1995. - V. 91, - No 2. - P. 580-615.
34- Cross C.E., De Lucia A.J., Reddy A.K., Hussain M.Z., Chow C.K. Ozone interactions with lung tissue. Biochemical approaches // Am J Med. - 1976. - V. 60, - No 7. - P. 929-935.
35- Halliwill J.R., Morgan B.J., Charkoudian N. Peripheral chemoreflex and baroreflex interactions in cardiovascular regulation in humans. J Physiol // - 2003. - No. 552. - P. 295-302. CAS PubMed Web of Science® Google Scholar.
36- Agadjanyin N.A., Kupriyanov S.V. Role of chemoreceptors in the zone of vertebral arteries in the formation of the cardiorespiratory functional system // Bulletin of Experimental Biology and Medicine. - 2009. - V. 148, - No 1. - P. 1-4.
37- Zanardi I., Borrelli E., Valacchi G., Travagli V., Bocci V. Ozone: a multifaceted molecule with unexpected therapeutic activity // Curr Med Chem. - 2016. - No 23. - P. 304-314.