Molecular study on gene and protein expression analysis of TGF beta1 - An in vitro model to develop drugs towards dentin tissue remodelling

Main Article Content

Anjali Sankar
Sindhu Ramesh
Selvaraj Jayaraman
Nishitha Arun

Keywords

Gene expression,Protein expression,TGF beta1, Dentin tissue remodelling

Abstract

Introduction: TGF-1–3 are one-of-a-kind multifunctional growth factors.TGF-beta1 is active in the reconstruction of the dental pulp tissue, according to recent reports. Furthermore, dental pulp cells can divide into odontoblast-like cells, which will then develop reparative dentine.
Materials and Methods: DEMM were used to culture and preserve 3T3 cells. The cells were incubated with LPS after differentiation. 3t3 Cells were used to extract total RNA. It was necessary to amplify cDNA. Using specific reagents, Rt PCR was used to measure the genes of interest.
Results: TGF-beta1 mRNA has a role in protein expression in lipopolysaccharide (LPS) induced macrophage-like cells.TGF-β signals are largely related to the magnitude and duration and careful regulation of their ligand activation, both temporally and spatially. If appropriately activated, TGF-β signalling plays an important function in tissue remodelling.
Conclusion: TGF-beta1 mRNA has a role in protein expression in lipopolysaccharide (LPS) induced macrophage-like cells.TGF-β signals are largely related to the magnitude and duration and careful regulation of their ligand activation, both temporally and spatially. If appropriately activated, TGF-β signalling plays an important function in tissue remodelling. The evidence suggests that hVEGF has a therapeutic potential in regeneration and other disorders of the pulp.

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