Identification of sexual assault cases by using RSID kit and STR of suspects and victim in some cases in Iraqi population

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Huda Salman Alagely
Mohammed Muayad Ahmed
Abeer Salman Alajely
Eman Noaman Ismail
Sanar Salman Alagely


STR, Power plex21 , RSID


In the Current study case, the methods for forensic identification of semen was using the Rapid Stain Identification (RSID-Semen). We demonstrate that RSID-Semen is accurate, reproducible, and highly sensitive for human fluid. The sensitivity of RSID allows investigators to sample a fraction of a questioned stain while retaining the majority for DNA-STR analysis. We demonstrate that RSID
identifies Semen from the clothes of victim and from suspects .RSID is a useful forensic test for determining which evidentiary items contain target sample and thus may yield a DNA profile by using power plex21 for the FTA of victim and suspect A ,B ,C comparison of DNA finger print for all profiles to make match between them

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1. Asia A. ;Mahdi M. Arsheed ; Sabbah D. ;Ali H.; Sura N. Hameed ;Aula A. Hussein (2018) . Molecular Analysis of Touched DNA Samples
Collected From Pistol Surfaces . Journal of Biotechnology Research Center . Vol. 12 No.1
2. Kayser M and Sajantila A (2001) Mutations at YSTR loci: implications for paternity testing and forensic analysis. Forensic Science Int.118, 116–121.
3. Kwok P Y (2011) Methods for genotyping single nucleotide polymorphisms. Annu. Rev. Genomics and Human Genetics 2, 235–258.
4. Liu Y, Zhang W, Jia Y S, Zhang F, Zhou K, Mei D and Huang X (2018) Multistep microsatellite mutation in a case of non-exclusion parentage.
Forensic Sci. Int. Genet. 16, 205-207.
5. Lu D, Liu Q, Wu W and Zhao H (2012) Mutation analysis of 24 short tandem repeats in Chinese Han population. Int. J. Legal Med.126, 331-335.
6. Ronaghi M (1996) Real-time DNA sequencing using detection of pyrophosphate release. Anal. Biochem. 242, 84–89.
7. Sun H, Liu S, Zhang Y and Whittle M R (2014) Comparison of southern Chinese Han and Brazilian Caucasian mutation rates at autosomal
short tandem repeat loci used in human forensic genetics. Int. J. Legal Med. 128, 1-9.
8. S. Vuichard, U. Borer, M. Bottinelli, C. Cossu,N. Malik, V. Meier, C. Gehrig, A. Sulzer, M.L.Morerod, V. Castella Differential DNA extraction of challenging simulated sexualassault samples: A Swiss collaborative study Investig. Genet., 2 (2011), p. 11, 10.1186/2041-2223-2-11
9. M.M. Ewing, J.M. Thompson, R.S. Mclaren, V.M. Purpero, K.J. Thomas, P.A. Dobrowski, G.A.Degroot, E.L. Romsos, D.R. Storts Human DNA
quantification and sample quality assessment: Developmental validation of the PowerQuant® system Forensic Sci. Int. Genet., 23 (2016),
pp. 166-177, 10.1016/j.fsigen.2016.04.007
10. F.C. Delfin, B.J. Madrid, M.P. Tan, M.C.A. De Ungria Y-STR analysis for detection and objective confirmation of child sexual abuse Int.
J. Legal Med., 119 (2005), pp. 158-163, 10.1007/s00414-004-0503-y
11. M. Kayser Forensic use of Y-chromosome DNA: a general overview Hum. Genet., 136 (2017), pp. 621-635, 10.1007/s00439-017-1776-9
12. L. Roewer Y-chromosome short tandem repeats in forensics—Sexing, profiling, and matching male DNA Wiley Interdiscip. Rev. Forensic
Sci. (2019), p. e1336, 10.1002/wfs2.1336
13. P. Gill, C.H. Brenner, B. Brinkmann, B. Budowle, A. Carracedo, M.A. Jobling, P. DeKnijff, M.Kayser, M. Krawczak, W.R. Mayr, N. Morling,
B. Olaisen, V. Pascali, M. Prinz, L. Roewer, P.M. Schneider, A. Sajantila, C. Tyler-Smith DNA Commission of the International Society of
Forensic Genetics: recommendations on forensic analysis using Y-chromosome STRs Forensic Sci. Int., 124 (2001), pp. 5-10, 10.1016/s0379-
14. Taylor, J.A. Bright, C. McGoven, C. Hefford, T.Kalafut, J. Buckleton Validating multiplexes for use in conjunction with modern interpretation
strategies Forensic Sci. Int. Genet., 20 (2016), pp. 6-19, 10.1016/j.fsigen.2015.09.011
15. P. Gill, J. Buckleton A universal strategy to interpret DNA profiles that does not require a definition of low-copy-number, Forensic Sci Int.
Genet., 4 (2010), pp. 221-227, 10.1016/j.fsigen.2009.09.008
16. D. Taylor, J.A. Bright, J. Buckleton The interpretation of single source and mixed DNA profiles Forensic Sci. Int. Genet., 7 (2013),
pp. 516-528, 10.1016/j.fsigen.2013.05.011
17. J.J.R.B. Rodriguez, J.M. Salvador, G.C. Calacal, R.P. Laude, M.C.A. De Ungria Allele frequencies of 23 autosomal short tandem repeat
loci in the Philippine population Leg. Med., 17 (2015), pp. 295-297, 10.1016/j.legalmed.2015.02.005
18. J.M. Salvador, J.J.R.B. Rodriguez, L.C.D.L. Car andang, A.B. Agmata, M.L.D. Honrado, F.C. Delfin, M.C.A. De Ungria Filipino DNA Variation
at 36 Y-chromosomal Short Tandem Repeat (STR) Marker UnitsPhilipp. J. Sci., 148 (S1) (2019), pp. 43-52