ESTIMATION OF THE LEVEL OF WHOLE SALIVARY CYTOKINES BEFORE AND AFTER REMOVAL OF FAULTY PROSTHESIS - AN IN-VIVO COMPARATIVE STUDY
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Keywords
Dental Prosthesis Failure; Saliva; Cytokine; Interleukin 6; Tumor Necrosis Factor Alpha; Salivary Biomarker; Oral Inflammation
Abstract
Materials and Methods:This in vivo comparative study was conducted in the Department of Prosthodontics, Dr. Ziauddin Ahmad Dental College, AMU in collaboration with the Department of Biochemistry, JNMC, AMU. A total of 62 patients aged 40-60 years with faulty prosthesis for more than one month were randomly selected. Exclusion criteria included smoking, systemic diseases, anti-inflammatory or antibiotic therapy within three months, and pregnancy. Whole unstimulated saliva samples were collected following standardized protocols (2-hour fasting, mouth rinsing, 10-minute waiting period, and 5-minute collection). Samples were centrifuged at 4°C for 10 minutes and stored at -20°C until analysis. IL-6 and TNF-α levels were estimated using analyzing kits. Statistical analysis was performed using paired samples t-test.
Results:A statistically significant reduction in both cytokine levels was observed in the overall study population following prosthesis removal. IL-6 levels decreased from a mean of 51.76 ± 39.21 pg/mL to 14.97 ± 14.82 pg/mL (t=8.82, p<0.001). TNF-α levels reduced from 98.58 ± 59.77 pg/mL to 43.21 ± 15.14 pg/mL (t=7.76, p<0.001). However, gender-stratified analysis showed non-significant reductions in both male and female subgroups individually.
Conclusion:Removal of faulty prosthesis results in a significant reduction in salivary IL-6 and TNF-α levels, indicating resolution of oral inflammation. Salivary cytokine analysis serves as a valuable, non-invasive tool for monitoring inflammatory changes following prosthetic rehabilitation. Timely intervention to replace faulty prostheses may contribute to reducing both local and systemic inflammatory burden.
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Prof. Geeta Rajput
(MDS Prosthodontics), Department of Prosthodontics, Crown and Bridge, Dr Ziauddin Ahmad Dental College and Hospital.
Dr. Shua Aftab
(MDS Prosthodontics), Department of Prosthodontics, Crown and Bridge, Dr Ziauddin Ahmad Dental College and Hospital.
Dr. Nikhil Menghani
(MDS Prosthodontics), Department of Prosthodontics, Crown and Bridge, Dr Ziauddin Ahmad Dental College and Hospital.
Dr. Shagufta Moin
Department of Biochemistry, Jawaharlal Nehru Medical College and Hospital.
Dr. Sabzar Abdullah
(MDS Prosthodontics), Department of Prosthodontics, Crown and Bridge, Dr Ziauddin Ahmad Dental College and Hospital.
Dr. Amina
(MDS Prosthodontics), Department of Prosthodontics, Crown and Bridge, Dr Ziauddin Ahmad Dental College and Hospital.
