Toxoplasmosis and Relation with Some Immunological Markers for Aborted Women in Thi-Qar Province

Main Article Content

Ibtisam Al Aboosi
Amall Y.AL-Mulla
Mustafa mudhafar
Qais R. Lahhob
Moamin Ibraheem Kate
Mohammed Hassib Ali
Murtadha A Al‑khegane
Mustafa Jawad Kadham
Abdulrahman Abbas Jasim


Toxoplasma gondii, Abortion, IFN-γ, CD4.


Background: Toxoplasma gondii is a major cause of abortion in pregnant women. Toxoplasmosis infection in the mother during pregnancy is frequently associated with transplacental transmission of the parasite to the fetus. The purpose of this study was to determine the serum level concentrations of (CD4 and IFN-)in patients with toxoplasmosis and control groups, aims of this study determine the serum level concentration of (CD4, IFNγ) in patients with toxoplasmosis and control groups. and compare the different concentrations of (CD4, IFN) in acute, chronic, and control groups.
Materials and MethodsFrom September 2020 to May 2021, a case-control study was carried out in ThiQar province to estimate the role of Toxoplasmosis in the occurrence of abortion among pregnant women. The current study included 120 aborted women as well as 20 healthy women as controls (Non pregnant and have no clinical history of abortion). All of these cases involved only females of reproductive age (16-44 years). Toxo-IgM and Toxo-IgG antibodies were tested first with a latex agglutination test (LAT) to detect positive samples, followed by an Enzyme Linked Immunesorbent Assay (ELISA) to detect IgG and IgM antibodies in both groups. CUSABIO method for measuring human interferon (IFN-) and 2- human cluster of differentiation 4 (CD4) (USA).
Results: IFN-γ values of a patient with Toxo- IgG, and Toxo- IgM were (31.4&38.64) respectively which is significantly higher than the control value )28.80( CD4 value in a patient with IgM was (3.045) which is significantly higher than the control patient value (2.925). Also, CD4 value of patients with Toxo IgG (4.457) was higher than control with a significant value.
Conclusion: The concentration of IFN-Y was increase in acute infection and decrease in chronic of toxoplasmosis . The concentration of CD4 were increased with chronic infection compared with acute.

Abstract 585 | pdf Downloads 136


Aghwan, S. S .; Al-Taee, A. F .; & Suliman, E. G. (2010). Detection of Toxoplasma gondii infection in domestic rabbits by using multiple techniques. Iraqi Journal of Veterinary Sciences, 24(2): 65–69.
Al-Ddory, A. R. (2011). Seroepidemiological Study of Toxoplasmosis among pregnant women in Salah-Adden government. Tikrit Medical Journal 2011; 17(1):64-73.
Al-Ghurairy, I .J .A. Serological epidemiological study of toxoplasmosis in Diyala province, Iraq. M.Sc. Thesis, University of Diyala, College of Education, 2007, 94pp.(in Arabic).
Al-Khafajy, A. H. M. (2004). Cytogenetic, Immunological and Biochemical Studies on Women Infected with Toxoplasma gondii with a history of abortion. M. Sc. thesis. College of Medicine, Al-Nahrain University, Baghdad, Iraq.
Al-Khashab, F. M. B. (2009). Isolate, diagnose the parasite Toxoplasma gondii and the study of its effects pathological and immunological (Doctoral dissertation, Ph. D. thesis).
AL-Mayahi J.R.C., (2011) Epidemiological study on Toxoplasma gondii in aborted women in Kut city. M.Sc. Thesis. College of Science.
University of Baghdad , 125pp.
AL-Taei N.H.J., (2015). Serological and immunological study to the prevalence of Toxoplasmosis among sewage workers, farm
workers and sellers of fruits and vegetables in Thi-Qar province – Iraq. M.Sc. Thesis, College of Sciences ,Thi-Qar University,2.
Aziz, F. M.; and Drueish, M. J. (2011). Toxoplasmosis: Serious disease during pregnancy. Baghdad Science Journal, 8(1): 91-95
Coombs, R. S.; Blank, M. L.; English, E. D.; Adomako-Ankomah, Y., Urama, I. C. S.; Martin, A. T.; & Boyle, J. P. (2020). Immediate interferon gamma induction determines murine host compatibility differences between Toxoplasma gondii and Neospora caninum. Infection and immunity, 88(4):17-20.
Dubey, Jitender P. (2020). The history and life cycle of Toxoplasma gondii. In Toxoplasma gondii. Academic Press, 25(3) 1–19
Fallahi Sh., Badparva E., Mohammadi M.,Ebrahimzadeh F. & Pournia Y., (2009).
Seroepidemiological study of Toxoplasmosis gondii in women referred to Khorramabad laboratory of health center for medical
examination before marriage, Lorestan province, Iran,2008.Asian journal of biological sciences,,2(3):88-94
Hafedh, A. A. A.; Ali, I. F.; & Alrikaby, N. J. A. (2020). Immunoregulation of cytokine signalling network in Toxoplasma gondii
infected-women. Annals of Tropical Medicine and Public Health, 23(1):23-114.
Hussain, M. A.; Stitt, V., Szabo, E. A.; & Nelan, B. (2017). Toxoplasma gondii in the Food Supply. Pathogens, 6(2):21.
Juma A. S. & Salman S., (2011).Correlation between apoptosis and Toxoplasma in abortion induction : Relevance of caspase 8.Int. J. Med.
Sci. , 3(6):181-192.
Kadir, M. A.; Ayla Kh. Ghalib; Nazakat F. Othman; Iman S. Ahmed (2011). Seroprevalence of Toxoplasma gondii among
Pregnant Women in Kirkuk / Iraq. Journal of Kirkuk University for Scientific Studies, 6(2):1-11.
Karem, L. O.( 2007). Seroepidemiological study of Toxoplasma gondii for aborted women sera in Sulaimania city. M.Sc. Thesis, College of
,Science, University of Baghdad., 124pp
Limon, G.; Beauvais, W.; Dadios, N.; Villena, I.; Cockle, C.; Blaga, R.; & Guitian, J. (2017). Crosssectional study of Toxoplasma gondii infection in pig farms in England. Foodborne Pathogens and Disease, 14(5): 269–281.
MacMicking, J. D. (2012). Interferon-inducible effector mechanisms in cell-autonomous immunity. Nature Reviews Immunology, 12(5): 367–382.
Roberts F.; Mets M.; Ferguson N.&Grady R.,(2001). Histopathological features of ocular toxoplasmosis in fetus and infant. J. Opthal.,,
119 (1): 51-58.
Sasai, M.; Pradipta, A.;& Yamamoto, M. (2018). Host immune responses to Toxoplasma gondii. International immunology, 30(3): 113-119.