Detection of the bacterial activity of saponins and some mineral elements in the local aqueous extract of licorice

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Zainab Hashim Mohammed
Nuha Ali Hadi al-Samarrai
Rabeah T . Mahmood


color reagents, aqueous extract, saponins, elements , Serratia, Klesiella Pneumoniae


The aim of the study was to detect and quantify saponins in the aqueous extract of licorice using HPLC technique. It was found that it contained the highest recorded concentration of Rhaoglucoglycyrrhizin in 335.37 µg/ml, while the lowest recorded concentration of Glycyrrhizin-20 methanoate µg /ml was 89.6. Also, the percentage of saponins in the aqueous and alcoholic extracts was 6.58% and 4.15%, respectively. The concentration of flavonoids and tannins in the aqueous extract of licorice were 18.99 and 6.28 ppm, respectively. The study also aimed to find the percentage of some major and minor metallic elements, including Fe, Na, Ca, K, Mg, Cu, Zn, Mn, and they were (1.382%, 2.04%, 5.5%, 5.38%, 4.186%, 1. 877%, 1.2%, 3.2% ) respectively. The effect of the extract containing the saponins mentioned above was studied in two bacterial species (Serratia, Klesiella Pneumoniae) using brain diffusion agar and then the added concentrations (100, 50,25,12.5)mg / Ml of the extract. The transparent zone of the bacterial growth inhibitor
showed a concentration of 100 mg / ml inhibitory zone for Klesiella Pneumoniae and 27 mm for Serratia .

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