DETECTION OF METALLO BETA LACTAMASE PRODUCTION AMONG ENTEROBACTERIACEAE ISOLATES RECOVERED FROM CLINICAL SPECIMENS IN A TERTIARY CARE IN CENTRAL INDIA
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Keywords
Enterobacteriaceae, mCIM, Metallo-Beta-Lactamase Producer, Minimum Inhibitory Concentration, Double Disk Synergy Test, E-test, IPM-Double Disc test., Metallo B-lactamase, carbapenum resistant, Antimicrobial resistant, Phenotypic detection
Abstract
Introduction: Recent observations have shown a rise in reports of metallo-beta-lactamase (MBL) producing isolates within the Enterobacteriaceae family. This alarming trend highlights a critical concern in the medical field: the diminishing number of effective therapeutic options for treating infections caused by these resistant organisms. As MBL enzymes enable bacteria to evade the effects of an array of beta-lactam antibiotics, healthcare providers face increasingly challenging scenarios in managing infections, underscoring the urgent need for novel treatment strategies and heightened surveillance.
Materials & Methods: This study was conducted in the Department of Microbiology at Index Medical College, Hospital & Research Centre in Indore, M.P. A total of 200 isolates of Enterobacteriaceae were included in this study. Resistance to imipenem was determined in isolates by disc diffusion and the minimum inhibitory concentration (MIC) method. Imipenem-resistant isolates were tested for MBL production by the Double Disk Synergy Test, MRP-EDTA IMP-EDTA combined disc test, and E-test.
Results: out of 200 isolates, 37(44.6%) were MBL-producing Enterobacteriaceae strains. Among which, E. coli (20, 24.1%) was the most prevalent organism, followed by K. pneumoniae (14, 16.9%), and Citrobacter Spp. (3, 3.6%).
Conclusion: This study demonstrated a high prevalence of MBL-producing Enterobacterales alongside widespread multidrug resistance, highlighting the need for regular surveillance and strict infection control measures to limit the dissemination of resistant strains.
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Shubham Shekhawat
Ph.D. Scholar, Dept. of Medical Microbiology, Malwanchal University, Indore, MP
Dr. Munesh Kumar Sharma
Professor Dept. of Microbiology, Amaltas Institute Of Medical Sciences Dewas, MP
