ISOLATION AND MOLECULAR IDENTIFICATION OF L-ASPARAGINASE PRODUCING FUNGI FROM SOIL
Main Article Content
Keywords
............
Abstract
L-asparaginase (L-asparagine amido hydrolase, E.C.3.5.1.1) is an extra cellular enzyme that has received considerable attention since it is used as an anticancer agent as well as for industrial application especially to reduce the acrylamide formation in fried foods. In the present study, the fungal isolates from rhizosphere soils were screened for the L-asparaginase production by using modified Czapek Dox agar containing L-asparagine and phenol red as indicator. The strain isolated from rhizosphere soil of Ipomoea muricata showed the maximum zone diameter of 1.05 cm. The 16s rDNA sequence analysis indicated that the strain was most closely related to Fusarium equiseti (99% similarity) and the 766 nucleotide sequences has been deposited to the GenBank (National Centre for Biotechnological Information, USA, with accession number JN400528).
References
2. Bridge, P., and B.Spooner. (2001) Soil fungi: diversity and detection. Plant Soil, 232: 147-154.
3. Carlile MJ. (1994).The success of the hypha and mycelium. In: The Growing Fungus (Gow NAR, Gadd GM (Eds)). London Chapmann and Hall. 3-19.
4. Rowlands, R.T. (1984) Industrial strain improvement: Mutagenesis and random screening procedures Enzyme and Microbial Technology, 6(1): 3–10
5. Alabouvette, C. (1990). Biological control of Fusarium wilt pathogens in suppressive soilsIn D. Hornby (ed.), Biological control of soil-borne pathogens. CAB International, Wallingford, 27 -34
6. Lodge, D.J., Fisher, P.J. and Sutton, B.C. (1996). Endophytic fungi of Manilkara bidentata leaves in Puerto Rico. Mycologia 88: 733-738.
7. Sette LD, Passarini MRZ, Delarmelina C. Salati F, Duarte MCT (2006). Molecular characterization and antimicrobial activity of endophytic fungi from coffee plants. World J. Microbiol. Biotechnol., 22: 1185-1195.
8. Crous, P.W., Braun, U., Schubert, K. and Groenewald, J.Z. (2007). Delimiting Cladosporium from morphologically similar genera. Studies in Mycology 58: 33-56.
9. Zhang, Y., Fournier, J., Pointing, S.B and Hyde, K.D. (2008). Are Melanomma pulvis-pyrius and Trematosphaeria pertusa congeneric? Fungal Diversity 33: 47-60.
10. Woese, C. R., E. Stackebrandt, T. J. Macke, and G. E. Fox (1985). A phylogenetic definition of the major eubacterial taxa. Syst. Appl. Microbiol. 6:143–151.
11. Woese, C. R. 1987. Bacterial evolution. Microbiol. Rev. 51:221–271.
12. Bottger, E. C. (1989). Rapid determination of bacterial ribosomal RNAsequences by direct sequencing of enzymatically amplified DNA. FEMS Microbiol.Lett.65, 171–176.
13. Palys, T., L. K. Nakamura, and F. M. Cohan. (1997). Discovery and classificationof ecological diversity in the bacterial world: the role of DNsequence data. Int. J. Syst. Bacteriol. 47:1145–1156.
14. Kolbert, C. P. and D. H. Persing. (1999) Ribosomal DNA sequencing as a tool for identification of bacterial pathogens. Curr. Opin. Microbiol. 2: 299–305.
15. Garrity, G. M., and J. G. Holt. (2001). The road map to the manual,In G. M. Garrity Bergey’s manual of systematic bacteriology. Springer-Verlag, New York, N.Y.119–166.
16. Harmsen, D., and H. Karch. (2004) 16S rDNA for diagnosing pathogens: a living tree. ASM News 70:19–24.
17. Leslie C. Lane (1978) A simple method for stabilizing protein-sulfhydrylgroups during SDS-gel electrophoresis, Analytical Biochemistry, 86(2): 655–664.
18. Bosshard PP, Abels S, Zbinden R, et al. (2003). Ribosomal DNA sequencingfor identification of aerobic gram-positive rods in the clinical laboratory(an 18-month evaluation). J Clin Microbiol; 41: 4134–40.
19. Clarridge JE (2004). Impact of 16S rRNA gene sequence analysis for identificationof bacteria on clinical microbiology and infectious diseases.Clin Microbiol Rev; 17: 840–62.
20. Drancourt D, Berger P, Raoult D. (2004). Systematic 16S rRNA gene sequencingof atypical clinical isolates identified 27 new bacterial species associated with humans. J Clin Microbiol; 42: 2197–202.
21. Leif Anderson, C. Carl Gahmberg, G. Martti Simes, A. Lasse Teerenhovi and Pekka Vuopio (l979) J. Cancer, 23: 306-311.
22. Ian C. Anderson Pamela I. Parkin (2007) Journal of Microbiological Methods 68, 2, 248-253.
23. Gulati, R., R.K. Saxena and R. Gupta, (1997). A rapid plate assay for screening L-asparaginase producing micro-organisms. Lett. Applied Microbiol., (24) 23-26.
24. Lapmak K, Lumyong S, Thongkuntha S, Wongputtisin P, Sardsud U. (2010). Chinag Mai J. Sci, 37: 160–164.
25. Nakhama K., Imada A., Igrasi S. and Tubaki K. (1973) J. Gen.Microbiol.75: 269-276.
Article Sidebar
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
All articles published in JPTCP are licensed under Copyright Creative Commons Attribution-NonCommercial 4.0 International License.
Dakshayani S S
Department of Biotechnology, UCS, Tumkur University, Tumkur-572103, Karnataka, India.
Marulasiddeshwara M B
Department of Studies and Research in Organic Chemistry, Tumkur University, Tumkur-572103, Karnataka, India.
Department of Studies and Research in Chemistry, UCS, Tumkur University, Tumkur-572103, Karnataka, India.
Rashmi Hosamani
Department of Microbiology, University College of Science, Tumkur University, Tumkur. 572103, Karnataka, India.
