DETECTION OF IMPORTANCE OF RIFAMPICIN AND ISONIAZID RESISTANCE AND DETERMINE RATE OF MDR-TB IN POSITIVE SMEAR SPUTUM SAMPLES FROM A TERTIARY CARE HOSPITAL OF M.P
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Keywords
InhA, katG, rpoB, LPA, PCR.
Abstract
One of the most effective strategies used to control tuberculosis is anti-tubercular therapy. In india various studies are reported earlier, the prevalence rate of MDR-TB very from region to region. Therefore , we determine rate of multi drug resistant –TB, Isoniazid and Rifampicin Mono resistance and common mutation pattern associated with them from our area using Genotype MTBDR plus assay in order to provide better patient care and reduce rate of Multi Drug Resistance –TB. This was a cross sectional study comprising of 800 sputum samples collected from DOTS center and processed by ZN staining and LPA. out of 800 sputum sample, 168 were smear positive . rate of MDR was found 9.88%. commonest mutation pattern seen was S531L in rifampicin and S315T1 in isoniazid. We found there is a high rate of INH mono resistance which was not being detected till now from this area and we also found, there is unrelated risk of isoniazid and rifampicin mono resistance so, inference of MDR based on RIF mono-resistance is also an inaccurate strategy to manage patients and drug sensitivity should be performed for both first line drug before stating MDR.
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Shivani Gour
Ph.D. Scholar, Department of Microbiology Index Medical & Research centre, Indore, M.P. India, Email: shivanigaur695@gmail.com
Dr Kailash Jatav
Associate Professor, Department of Microbiology, Index Medical College, Indore, M.P. Email: Kailasha099@gmail.com
Dr Versha Rajput
Assistant Professor, Department of Paramedical, Teerthanker Mahaveer University, Moradabad, U.P. Email: varsharajput52010@gmail.com
