ECOLOGY AND MOLECULAR CHARACTERIZATION OF SOL FISH (CHANNA MARULIUS) IN DISTRICT SWABI, KHYBER PAKHTUNKHWA, PAKISTAN
Main Article Content
Keywords
Sol Fish, Channa marulius, Habitat, Distribution, Genomic analysis, Pakistan
Abstract
Channa marulius commonly known as Sol fish is distributed across various countries of the south Asian subcontinent. The natural habitat of Channa marulius includes standing or slow-moving water in swamplands, water channels, lochs and deep water lagoons in rivers. These carnivorous fishes thrive in environments rich in submerged aquatic vegetation. The study was conducted to study the ecology and molecular characterization of Channa marulius in district Swabi, Khyber Pakhtunkhwa, Pakistan. The ecological factors such as water temperature and its quality play vital role in the distribution and population density of Channa marulius. Water quality parameters such as dissolved oxygen (DO), pH and availability of prey fauna are closely linked to its distribution, population density and its survival rates. A total of 200 fish specimens were collected by using hand nets, dip net and cast net from five different locations of Swabi (Garh-munara, Batakra, Zarobai, Hund and Allader). Morphometric parameters such as total length, head length and body depth were measured while meristic traits including dorsal and anal fin rays, lateral line scales and ventral fin rays were also recorded. Blood and tissues samples were collected from each fish for further genomic analysis. DNA was extracted and amplified using PCR with species-specific primers. Molecular characterization was performed by sequencing mitochondrial Cytochrome Oxidase subunit I (COI) gene. Two species of genus Channa named Channa marulius and Channa punctatus were identified. Phylogenetic analysis confirmed the presence of Channa marulius in district Swabi with 97% similarity with the sequences of the Channa marulius from other countries validating its classification and regional presence. The results highlight the ecological significance and growing demand for Channa marulius. This study provides a foundational molecular database for Channa marulius, supporting future research on genetic aspects of this fish species, its conservation strategies and aquaculture development in the region of Khyber Pakhtunkhwa Pakistan.
References
2. Ali, F.S., Ismail, M. and Aly, W., 2020. DNA barcoding to characterize biodiversity of freshwater fishes of Egypt. Molecular Biology Reports, 47, pp.5865-5877. https://link.springer.com/article/10.1007/s11033-020-05657-3
3. Barman, S.K., Das, B.R., Kunda, M., Mazumder, S.K., Uddin, M.J. and Barman, P.P., 2023. Length-weight relationships and condition factors of mono-and mixed-sex Nile tilapia (Oreochromis niloticus) in open water cage culture system. Journal of Fisheries, 11(1), pp.111204-111204. DOI: https://doi.org/10.17017/j.fish.431
4. Bhatti, E.M., Qureshi, N.A., Rasool, F., Siddique, M. and Siddiqui, A.B., 2014. Characterization of Channa marulius populations in various water bodies of Punjab, Pakistan. Pakistan Journal of Agricultural Sciences, 51(3).
5. Dua, A. and Kumar, K., 2006. Age and growth patterns in Channa marulius from Harike Wetland (A Ramsar site), Punjab, India. Journal of environmental Biology, 27(2), p.377.
6. Fawad, M., Gul, B., Khan, H. and Khan, Z., 2013. Study and collection of hydrophytes of the district Swabi, Pakistan. Pakistan Journal of Weed Science Research, 19(4). https://www.researchgate.net/publication/327120197
7. Ghatee, M.A., Sharifi, I., Mirhendi, H., Kanannejad, Z. and Hatam, G., 2013. Investigation of double-band electrophoretic pattern of ITS-rDNA region in Iranian isolates of Leishmania tropica. Iranian journal of parasitology, 8(2), p.264. PMCID: PMC3724152
8. Hebert, P.D., Cywinska, A., Ball, S.L. and DeWaard, J.R., 2003. Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B: Biological Sciences, 270(1512), pp.313-321. https://doi.org/10.1098/rspb.2002.2218
9. Hossen, M.A., Paul, A.K., Hossain, M.Y., Ohtomi, J., Sabbir, W., Rahman, O., Jasmin, J., Khan, M.N., Islam, M.A., Rahman, M.A. and Khatun, D., 2019. Estimation of Biometric Indices for Snakehead Channa punctata (Bloch, 1793) through Multi-model Inferences. Jordan Journal of Biological Sciences, 12(2).
10. Khan, A., Yousafzai, A.M., Latif, M., Rehman, A., Khan, Q., Zaib, A., Ullah, A., Sthanadar, A.A., Haq, I. and Aziz, A., 2014. Analysis of selected water quality parameters and heavy metals of Indus River at BekaSwabi, Khyber Pakhtunkhwa, Pakistan. International Journal of Biosciences (IJB), 4(2), pp.28-38. http://dx.doi.org/10.12692/ijb/4.2.28-38.
11. Khan, M.A., Miyan, K. and Khan, S., 2013. Morphometric variation of snakehead fish, C hanna punctatus, populations from three I ndian rivers. Journal of Applied Ichthyology, 29(3), pp.637-642. https://doi.org/10.1111/j.1439-0426.2012.02058.x
12. Kumar, S., Stecher, G., Li, M., Knyaz, C. and Tamura, K., 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular biology and evolution, 35(6), p.1547. Doi:10.1093/molbev/msy096
13. Li, X., Musikasinthorn, P. and Kumazawa, Y., 2006. Molecular phylogenetic analyses of snakeheads (Perciformes: Channidae) using mitochondrial DNA sequences. Ichthyological Research, 53, pp.148-159. https://doi.org/10.1007/s10228-005-0321-3.
14. Mirza, M.R. and Sandhu, A.A., 2007. Fishes of the Punjab Pakistan.
15. Miyan, K., Afzal Khan, M. and Khan, S., 2014. Stock structure delineation using variation in otolith chemistry of snakehead, Channa punctata (Bloch, 1793), from three Indian rivers. Journal of Applied Ichthyology, 30(5), pp.881-886. https://doi.org/10.1111/jai.12479
16. Plamoottil, M., 2017. Taxonomic notes on Mystus species of northern Kerala. J Aquac Res Dev, 8, p.495. DOI: 10.4172/2155-9546.1000495
17. Rakhi, R.F., 2023. Investigating the Population Genetic Structure of the Endangered Great Snakehead (Channa marulius) in Open Waterbodies of Bangladesh Using Mitochondrial DNA Markers. Egyptian Journal of Aquatic Biology and Fisheries, 27(3), pp.979-995. DOI:10.21608/ejabf.2023.307494.
18. Rosmawati, Abustam, E., Tawali, A.B., Said, M.I. and Sari, D.K., 2018. Effect of body weight on the chemical composition and collagen content of snakehead fish Channa striata skin. Fisheries science, 84(6), pp.1081-1089. DOI: 10.1007/s12562-018-1248-8
19. Song, L.M., Munian, K., Abd Rashid, Z. and Bhassu, S., 2013. Characterisation of Asian snakehead murrel Channa striata (Channidae) in Malaysia: an insight into molecular data andmorphologicalapproach. TheScientificWorldJournal, 2013.https://doi.org/10.1155/2013/917506
20. Tamura, K. and Nei, M., 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular biology and evolution, 10(3), pp.512-526. https://doi.org/10.1093/oxfordjournals.molbev.a040023
21. Ward RD, Zemlak TS, Innes BH, Last PR, Hebert PD (2005) DNA barcoding Australia's fish species. Philosophical Transactions of the Royal Society B: Biological Sciences 360(1462): 1847–1857. https://doi.org/10.1098/rstb.2005.1716
22. Yadav, K.C., Mishra, A., Raizada, S., Sahu, V. and Srivastava, P.P., 2016. Influence of formulated diet on survival and growth of giant-snakehead, Channa marulius reared in pond condition. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 86, pp.97-103. https://doi.org/10.1007/s40011-014-0414-8
23. Yousafzai, A.M. and Khan, A., 2013. Fish diversity of Indus river at Beka Swabi, Khyber Pakhtunkhwa, Pakistan. International Journal of Biosciences (IJB), 3(12), pp.65-72.
24. Zhu, L.Y., Nie, L., Zhu, G., Xiang, L.X. and Shao, J.Z., 2013. Advances in research of fish immune-relevant genes: a comparative overview of innate and adaptive immunity in teleosts. Developmental & Comparative Immunology, 39(1-2), pp.39-62. https://doi.org/10.1016/j.dci.2012.04.001
25. Zhu, S.R., Fu, J.J., Wang, Q. and Li, J.L., 2013. Identification of Channa species using the partial cytochrome c oxidase subunit I (COI) gene as a DNA barcoding marker. Biochemical Systematics and Ecology, 51, pp.117-122. https://doi.org/10.1016/j.bse.2013.08.011
26. Zuraini, A., Somchit, M.N., Solihah, M.H., Goh, Y.M., Arifah, A.K., Zakaria, M.S., Somchit, N., Rajion, M.A., Zakaria, Z.A. and Jais, A.M., 2006. Fatty acid and amino acid composition of three local Malaysian Channa spp. fish. Food Chemistry, 97(4), pp.674-678. https://doi.org/10.1016/j.foodchem.2005.04.031
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
All articles published in JPTCP are licensed under Copyright Creative Commons Attribution-NonCommercial 4.0 International License.
Maryam Begum
Department of Zoology, Abdul Wali Khan University Mardan 23200, Khyber Pakhtunkhwa, Pakistan.
Asad Ullah
College of Veterinary Sciences and Animal Husbandry (CVS & AH), Abdul Wali Khan University Mardan 23200, Khyber Pakhtunkhwa, Pakistan.
Yousaf Ali
Senior Scientific Officer, Precession System Training Center, PCSIR Peshawar 25120, Khyber Pakhtunkhwa, Pakistan.
Raheela Taj
Institute of Chemical Sciences (ICS), University of Peshawar 25120, Khyber Pakhtunkhwa, Pakistan.
Shumaila Gul
Department of Chemical and Life Sciences, Qurtuba University of Science and information Technology Peshawar, Khyber Pakhtunkhwa, Pakistan.
Sulha Syed
Sarhad Institute of Allied Health Sciences, Sarhad Universityof Science and Information Technology, Peshawar 25100, Khyber Pakhtunkhwa, Pakistan.
Imad Khan
College of Veterinary Sciences and Animal Husbandry (CVS & AH), Abdul Wali Khan University Mardan 23200, Khyber Pakhtunkhwa, Pakistan.
Ali Gohar
College of Veterinary Sciences and Animal Husbandry (CVS & AH), Abdul Wali Khan University Mardan 23200, Khyber Pakhtunkhwa, Pakistan.
Rainaz Begum7
Department of Chemistry, Abdul Wali Khan University Mardan 23200, Khyber Pakhtunkhwa, Pakistan.
Tahira Tayyeb
Department of Zoology, Abdul Wali Khan University Mardan 23200, Khyber Pakhtunkhwa, Pakistan.
Mansoor Ahmad
Department of Zoology, Abdul Wali Khan University Mardan 23200, Khyber Pakhtunkhwa, Pakistan.
Noor Us Saba Shujaat
Department of Zoology, Abdul Wali Khan University Mardan 23200, Khyber Pakhtunkhwa, Pakistan.
