ANTI-INFLAMMATORY, ANTI-DIABETIC AND LARVICIDAL ACTIVITY OF ETHYL ACETATE FRACTION OF LEAVES AND STEM OF VIBURNUM COTINIFOLIUM D.DON.
Main Article Content
Keywords
Larvicidal, anti-inflammatory, anti-diabetic, ethyl acetate.
Abstract
Vibernum cotinifolium is a deciduous shrub growing up to 3.5 m. The plant is traditionally used for many treatments. Viburnum cotinifolium is well known in folk medicine for sedative, spasmolytic, antioxidants, antibacterial, astringent and anti-asthematic properties. In current study the Larvicidal, anti-inflammatory and anti-diabetic activity of the ethyl acetate fraction of stem and leaves of Vibernum cotinifolium has been explored. In larvicidal activity the extracts were tested against Adies larvae and showed moderate to high toxic effect on Adies larvae after 72 hours of exposure at 300ppm concentration, while maximum inhibition (80%) was observed in ethyl acetate fraction of leaves of V. cotinifolium with LC50 value of 187.5 ppm. In stem ethyl acetate fraction the maximum inhibition was 71.7 percent after 72 hours of exposure at the concentration of 300ppm. In carragenan-induced paw edema model each fraction was supplied at a dose of 100 mg/kg, 200 mg/kg and 300 mg/kg. At 300 mg/kg the ethyl acetate fraction of leaves showed 54.83 % inhibition. While the stem fraction showed 49.82% inhibition. In anti-diabetic activity results were well comparable with the standard drug glibenclamide. The stem fraction was more effective as compared to standard drug with 86.07 percent inhibition. In all the three bioassays the plant showed potent inhibition which ensure its uses as cure for these diseases.
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Saima
Department of Botany, University of Peshawar, Pakistan.
Tanvir Burni
Department of Botany, University of Peshawar, Pakistan.
Rasool Khan
Institute of Chemical Sciences, University of Peshawar, Pakistan.
Muhammad Jamal
Department of Pharmacy, City University of Science and Information Technology, Pakistan.
Habib Ullah
Department of Zoology, University of Peshawar, Pakistan.