ANTIBACTERIAL EFFICACY OF MORINGA OLEIFERA LATEX EXTRACT AGAINST PYOGENIC BACTERIA
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Keywords
Moringa oleifera, Streptococcus pyogenes, pharyngitis, tonsillitis.
Abstract
Effective management of biofilm-related oral infectious diseases and increased resistance to antimicrobial agents and elevated virulence is now leading as a global challenge. Streptococcus pyogenes has been reported to be one of the most common and influential aerobic bacteria associated with leading cause of uncomplicated bacterial pharyngitis and tonsillitis. Due to prevailing use of antibiotics has been raising a real alarming issue, hence one alternative to overcome these problems is to use natural ingredients such as materials from plant extract such as Moringa oleifera latex. In the past, researchers paid only a little attention towards the plant extract which acts effectively against oral infection by latex.
This study is aimed to evaluate the antibacterial activity of the latex of Moringa oleifera against Streptococcus pyogenes from the provided pure cultured master plate. Latex ( 2gm latex – 2ml DMSO-Dimethyl sulfoxide) is given to dissolved with DMSO within 48 hrs of collection left undisturbed overnight. DMSO is previously tested for negative control.
Antibacterial Assay of Latex were calculated based on the inhibition zones using the Mueller–Hinton agar diffusion method (19.5–23.4 mm and 12–15 mm) , minimum inhibitory concentration (MIC) using Mueller–Hinton broth in a microdilution method ( 100% - 25%), and minimum bactericidal concentration (MBC) using nutrient broth and nutrient agar( 100%and 50%) .
On witnessing the inhibitory results of M. oleifera latex can be considered one of the new infection-fighting strategies in controlling pyogenic bacteria responsible for oral infections, but further in vivo research and discovery of the mode of its action are immediately required to shed the light on the effects of latex against infection in buccal cavity instead of taking antibiotic pills, which might save the world from drug resistant bacterial pathogen
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Amrita Roy
M. Sc microbiology, St Xavier's College, Kolkata (Autonomous),