IN-VITRO ANTI-INFLAMMATORY ACTIVITY OF SIDDHA FORMULATION PANCHATHIKTHA GHRITHAM BY PROTEIN (ALBUMIN) DENATURATION ASSAY

Abstract

Siddha medicine is a traditional system of healing that emphasizes the use of natural remedies for the management of various ailments. Rooted in ancient wisdom, it is believed to have been developed by the Siddhars, with particular applications in the prevention and treatment of childhood disorders. Among them Panchathiktha Ghritham which is mentioned in Siddha literature Chikicha rathnadeepam which is used for the management of psoriasis in children. The siddha formulation , composed of 23 ingredients, has been clinically evaluated, and the constituent drugs present in Panchathiktha Ghrithm have demonstrated the ability to regulate inflammatory responses, thereby showing therapeutic potential in the management of Kalanjagapadai ( Psoriasis in children). Method: The drug screened for Anti-inflammatory activity.

Results: The result obtained from the present study clearly indicates that the test drug Panthathiktha ghritham was effective in inhibiting heat induced albumin denaturation. Maximum percentage inhibition of about 39.24 ± 5.27 % was observed at 500μl, when compared to that Diclofenac sodium, a standard anti-inflammatory agent with the maximum inhibition 90.51 ± 1.70 % at the concentration of 100 μg/ml.

Conclusion: The results of the study revealed that the Siddha formulation Panchathiktha Ghritham complies with the established standards and may be considered for clinical management of Kalanjagapadai. However, further investigations focusing on its anti-inflammatory activity in the context of Kalanjagapadai are warranted. Based on the findings, it can be concluded that the test drug Panchathiktha Ghritham exhibits a promising anti-inflammatory effect, as demonstrated by the protein denaturation assay.