A REVIEW ON ANALYTICAL METHOD FOR ESTIMATION OF SIBUTRAMINE HYDROCHLORIDE AND TOPIRAMATE IN SYNTHETIC MIXTURE.
Main Article Content
Keywords
Anti-obesity drug, GABAergic activity, Weight management, HPLC, LC-MS
Abstract
Sibutramine hydrochloride and Topiramate are pharmacological agents with distinct therapeutic roles but a potential for complementary application. Sibutramine hydrochloride, a centrally acting serotonin and norepinephrine reuptake inhibitor, was primarily used as an anti-obesity agent due to its appetite-suppressing effects, though its use has declined owing to cardiovascular safety concerns. Topiramate, an anticonvulsant with multiple mechanisms including modulation of voltage-gated ion channels, enhancement of GABAergic activity, and inhibition of carbonic anhydrase, has been effectively employed in epilepsy, migraine prophylaxis, and weight management. Recent studies have explored their combined therapeutic potential in the management of obesity and related metabolic disorders, as Topiramate may enhance weight reduction and mitigate some adverse effects associated with Sibutramine. Analytical development for simultaneous estimation of these drugs is crucial for pharmaceutical formulation research, ensuring accuracy, precision, and regulatory compliance in accordance with ICH guidelines. The integration of pharmacological insights with validated analytical methods supports further investigation into their synergistic use and safe therapeutic application.
References
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9. Rosa F, Négrier P, Espeau P. Racemic compound and conglomerate of anhydrous sibutramine hydrochloride: a rare case of relative stability. CrystEngComm. 2016;18(36):6903-7.
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11. Abtahi MA, Abtahi SH, Fazel F, Roomizadeh P, Etemadifar M, Jenab K, Akbari M. Topiramate and the vision: a systematic review. Clinical Ophthalmology. 2012 Jan 12:117-31.
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13. Guglielmo R, Martinotti G, Quatrale M, Ioime L, Kadilli I, Di Nicola M, Janiri L. Topiramate in alcohol use disorders: review and update. CNS drugs. 2015 May;29(5):383-95.
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16. Khalil NY, AlRabiah HK, Al Rashoud SS, Bari A, Wani TA. Topiramate: Comprehensive profile. Profiles of drug substances, excipients, and related methodology. 2019 Jan 14;44:333-78.
17. Bai YF, Zeng C, Jia M, Xiao B. Molecular mechanisms of topiramate and its clinical value in epilepsy. Seizure. 2022 May 1;98:51-6.
18. Powers SW, Coffey CS, Chamberlin LA, Ecklund DJ, Klingner EA, Yankey JW, Korbee LL, Porter LL, Hershey AD. Trial of amitriptyline, topiramate, and placebo for pediatric migraine. New England Journal of Medicine. 2017 Jan 12;376(2):115-24.
19. Cherukuri S, Batchu UR, Mandava K, Cherukuri V, Ganapuram KR. Formulation and evaluation of transdermal drug delivery of topiramate. International journal of pharmaceutical investigation. 2017 Jan;7(1):10.
20. Manhapra A, Chakraborty A, Arias AJ. Topiramate pharmacotherapy for alcohol use disorder and other addictions: a narrative review. Journal of addiction medicine. 2019 Jan 1;13(1):7-22.
21. Gong ZS, Guo QY, Zhang J, Gong S, Liu F, Xu L. Uncertainty Evaluation for Determination of Sibutramine in Health Food by HPLC-MS/MS. Natural Product Communications. 2024 Oct;19(10):1934578X241292937.
22. Nguyen TN, Nguyen HK, Nguyen TN, Duong TN, Pham TT, Nguyen TK. Validation of sibutramine and phenolphthalein determination by HPLC-PDA in natural dietary supplements for body-weight reduction. Food Research. 2024 Dec;8(6):162-9.
23. Suparmi S, Yuliyanti S, Karyadini HW, Syamsudin AM, Gau EK. Toxicity of sibutramine hydrochloride-adulterated weight loss supplements in rats based on biochemical and organ weight parameters. Journal of Pharmacy & Pharmacognosy Research. 2024;12(2):363-70.
24. Sembiring BM, Fahdi F, Tanjung DA, Faisal B. Investigation of herbal medicine identified sibutramine hcl through analysis of thin layer chromatography and uv-vis spectrophotometry methods. Rasayan journal of chemistry. 2024 oct 1;17(4).
25. Chorilli M, Bonfilio R, da Silva Chicarelli R, Salgado HR. Development and validation of an analytical method by RP-HPLC for quantification of sibutramine hydrochloride in pharmaceutical capsules. Analytical Methods. 2011;3(4):985-90.
26. Kilicarslan G, Imamoglu E, Kucuk A, Özdemir A. UV spectrophotometric, derivative spectrophotometric and RP-HPLC-DAD determination of sibutramine. Reviews in Analytical Chemistry. 2010 Dec;29(3-4):169-96.
27. Imran MD, PATHADE P. Development and validation of stability indicating uv spectrophotometric method for the estimation of sibutramine hydrochloride monohydrate in bulk and capsule dosage form. Int J Pharm Pharm Sci. 2011;3(4):53-6.
28. Hwang YY, Shin DC, Nam YS, Cho BK. Characterization, stability, and pharmacokinetics of sibutramine/β-cyclodextrin inclusion complex. Journal of Industrial and Engineering Chemistry. 2012 Jul 25;18(4):1412-7.
29. Ayad M, Abdellatef H, Hosny M, Kabil N. Utility of molybdophosphoric acid in qualitative and quantitative analysis of Sibutramine HCl, Sumatribtan succinate and Lomefloxacine HCl. International Journal of Pharmaceutical and Biomedical Research. 2012;3(2):121-26.
30. Singh AK, Garca PL, Gomes FP, Yano HM, Auricchio MT, Kedor-Hackmann ER, Santoro MI. Development and validation of sensitive methods for determination of sibutramine hydrochloride monohydrate and direct enantiomeric separation on a protein-based chiral stationary phase. Journal of AOAC International. 2008 May 1;91(3):572-9.
31. Maghfirah R, Farida M, Purnama A, Afifi MR, MZ K, Rahmawati E. Quantitative Analysis of Sibutramine Hydrochloride in Slimming Herbal Medicines in Banda Aceh Using UV-Vis Spectrophotometry. BIOLOGICA SAMUDRA. 2025 Jun 30;7(1):54-62.
32. Simaremare ES, Susilowati RA, Astuti YD, Hermawan R, Gunawan E, Pratiwi RD. Analysis of acetaminophen, mefenamic acid, sibutramine hydrochloride, and sildenafil citrate. Journal of Applied Pharmaceutical Science. 2018 Nov 30;8(11):048-56.
33. Yenare SS, Tare M, Navasupe PS, Bangar AB, Bole SS. UV spectroscopic method for estimation of topiramate bulk and pharmaceutical dosage forms.
34. Pinto EC, Dolzan MD, Cabral LM, Armstrong DW, Sousa VP. Topiramate: a review of analytical approaches for the drug substance, its impurities and pharmaceutical formulations. Journal of chromatographic science. 2016 Feb 1;54(2):280-90.
35. Ibrahim FA, El-Yazbi AF, Wagih MM, Barary MA. Sensitive inexpensive spectrophotometric and spectrofluorimetric analysis of ezogabine, levetiracetam and topiramate in tablet formulations using Hantzsch condensation reaction. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2017 Sep 5;184:47-60.
36. El-Yazbi AF, Wagih MM, Ibrahim F, Barary MA. Spectrofluorimetric determination of topiramate and levetiracetam as single components in tablet formulations and in human plasma and simultaneous fourth derivative synchronous fluorescence determination of their co-adminstered mixture in human plasma. Journal of fluorescence. 2016 Jul;26(4):1225-38.
37. Khan FI, Rehman MT, Sameena F, Hussain T, AlAjmi MF, Lai D, Khan MK. Investigating the binding mechanism of topiramate with bovine serum albumin using spectroscopic and computational methods. Journal of Molecular Recognition. 2022 Jul;35(7):e2958.
38. Mafi Z, Sohrabi MR, Davallo M. Fast and facile UV-spectrophotometry-assisted chemometrics methods for the simultaneous determination of phenytoin and phenobarbital as antiepileptic drugs in combined dosage form and urine sample. Iran. J. Chem. Chem. Eng.(IJCCE) Research Article Vol. 2024 Sep 3;43(9).
39. Sunitha PG, Deattu N, Dhanalakshmi R, Gangatharan A, Kavi BA, Shankar GM, Sathya M. Colorimetric methods for the estimation of topiramate in tablets. J. Drug Deliv. Ther. 2016;6:11-3.
40. Nejdar P, Salunkhe S, Pishawikar S, Uchgaon K. Development of Validated RP-HPLC Method for Determination of Sibutramine Applying QbD Approach.
41. Nguyen TN, Nguyen HK, Nguyen TN, Duong TN, Pham TT, Nguyen TK. Validation of sibutramine and phenolphthalein determination by HPLC-PDA in natural dietary supplements for body-weight reduction. Food Research. 2024 Dec;8(6):162-9.
42. Kozhuharov VR, Chakarov D, Ivanova S, Ivanov K. Development and validation of a high-performance thin-layer chromatography method for detection of sibutramine in dietary supplements. Folia Medica. 2024 Apr 30;66(2):255-63.
43. Gürbüzer A, Ulusoy HI, Narin İ. Sensitive Detection of Sibutramine and Fluoxetine Adulteration in Herbal Slimming Products by Means of Using Magnetic Solid Phase Extraction Combined with HPLC–DAD. Food Analytical Methods. 2025 Mar;18(3):459-70.
44. Ping T, Zheng M, Zhang P, Yan T, Miao X, Wang K, Lian K. Determination of 12 anti-obesity drugs in human plasma by a 96-well protein precipitation plate using HPLC-MS. RSC advances. 2022;12(40):26016-22.
45. Simaremare ES, Susilowati RA, Astuti YD, Hermawan R, Gunawan E, Pratiwi RD. Analysis of acetaminophen, mefenamic acid, sibutramine hydrochloride, and sildenafil citrate. Journal of Applied Pharmaceutical Science. 2018 Nov 30;8(11):048-56.
46. Kimani M, Lanzarotta A, Batson J. Analysis of unlabeled sibutramine in dietary supplements using surface-enhanced Raman spectroscopy (SERS) with handheld devices.
47. Qin L, Wang X, Lu D. Quantitative determination and validation of topiramate and its tablet formulation by 1 H-NMR spectroscopy. Analytical Methods. 2019;11(5):661-8.
48. Salzmann L, Spescha T, Singh N, Kobel A, Fischer V, Schierscher T, Bauland F, Geistanger A, Risch L, Geletneky C, Seger C. An isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)-based candidate reference measurement procedure for the quantification of topiramate in human serum and plasma. Clinical Chemistry and Laboratory Medicine (CCLM). 2023 Oct 26;61(11):1942-54.
49. Kuczyńska j, zakrzewska-sito a, bochyńska a, sienkiewicz-jarosz h, dermanowski m, mierzejewski p. Development of method for determining topiramate in various biological matrices (plasma, saliva, hair) and its application in clinical practice. Acta Poloniae Pharmaceutica. 2024 Jan 1;81(1).
50. Thumbar H, Dhalani J, Patel H, Dhaduk B. In Situ Identification and Quantification of Genotoxic Sulfonyl Chloride Impurity in Topiramate via LC-MS/MS. MethodsX. 2025 Jun 14:103441.
2. Maluf DF, Farago PV, Barreira SM, Pedroso CF, Pontarolo R. Validation of an analytical method for determination of sibutramine hydrochloride monohydrate in capsules by UV-vis spectrophotometry. Latin American Journal of Pharmacy. 2007 Nov 1;26(6):909.
3. Maggadani BP, Amalina N. Determination of sibutramine adulterated in herbal slimming products using TLC densitometric method. Indonesian Journal of Pharmacy/Majalah Farmasi Indonesia. 2016 Jan 1;27(1).
4. Oberholzer HM, Van der Schoor C, Bester MJ. Sibutramine, a serotonin–norepinephrine reuptake inhibitor, causes fibrosis in rats. Environmental Toxicology and Pharmacology. 2015 Jul 1;40(1):71-6.
5. Shah MS, Patel ZK, Bharucha R, Talati T, Benz M, Talati TS. Sibutramine-induced nonischemic cardiomyopathy. Cureus. 2022 Jan 26;14(1).
6. Lee H. Data exclusivity through New Drug Reexamination in Korea: sibutramine hydrochloride (Reductil®) vs. sibutramine mesylate (Slimmer®) as an example. Translational and Clinical Pharmacology. 2018 Jun 18;26(2):49.
7. Yun J, Chung E, Choi KH, Cho DH, Song YJ, Han KM, Cha HJ, Shin JS, Seong WK, Kim YH, Kim HS. Cardiovascular safety pharmacology of sibutramine. Biomolecules & therapeutics. 2015 Jul 1;23(4):386.
8. Ouyang L, Jiang Z, Wang N, Zhu L, Tang H. Rapid surface enhanced Raman scattering (SERS) detection of sibutramine hydrochloride in pharmaceutical capsules with a β-cyclodextrin-Ag/polyvivnyl alcohol hydrogel substrate. Sensors. 2017 Jul 10;17(7):1601.
9. Rosa F, Négrier P, Espeau P. Racemic compound and conglomerate of anhydrous sibutramine hydrochloride: a rare case of relative stability. CrystEngComm. 2016;18(36):6903-7.
10. Kamardi t, fidrianny i, musadad a. Development of analytical method for identification of sibutramine hydrochloride in traditional medicine using solid phase extraction: high-performance liquid chromatography. Development. 2016;9(6).
11. Abtahi MA, Abtahi SH, Fazel F, Roomizadeh P, Etemadifar M, Jenab K, Akbari M. Topiramate and the vision: a systematic review. Clinical Ophthalmology. 2012 Jan 12:117-31.
12. Pearl NZ, Babin CP, Catalano NT, Blake JC, Ahmadzadeh S, Shekoohi S, Kaye AD. Narrative review of topiramate: clinical uses and pharmacological considerations. Advances in Therapy. 2023 Sep;40(9):3626-38.
13. Guglielmo R, Martinotti G, Quatrale M, Ioime L, Kadilli I, Di Nicola M, Janiri L. Topiramate in alcohol use disorders: review and update. CNS drugs. 2015 May;29(5):383-95.
14. Fariba KA, Saadabadi A. Topiramate. InStatPearls [Internet] 2024 Jun 8. StatPearls Publishing.
15. Cross JH, Riney CJ. Topiramate. The treatment of epilepsy. 2015 Oct 2:642-51.
16. Khalil NY, AlRabiah HK, Al Rashoud SS, Bari A, Wani TA. Topiramate: Comprehensive profile. Profiles of drug substances, excipients, and related methodology. 2019 Jan 14;44:333-78.
17. Bai YF, Zeng C, Jia M, Xiao B. Molecular mechanisms of topiramate and its clinical value in epilepsy. Seizure. 2022 May 1;98:51-6.
18. Powers SW, Coffey CS, Chamberlin LA, Ecklund DJ, Klingner EA, Yankey JW, Korbee LL, Porter LL, Hershey AD. Trial of amitriptyline, topiramate, and placebo for pediatric migraine. New England Journal of Medicine. 2017 Jan 12;376(2):115-24.
19. Cherukuri S, Batchu UR, Mandava K, Cherukuri V, Ganapuram KR. Formulation and evaluation of transdermal drug delivery of topiramate. International journal of pharmaceutical investigation. 2017 Jan;7(1):10.
20. Manhapra A, Chakraborty A, Arias AJ. Topiramate pharmacotherapy for alcohol use disorder and other addictions: a narrative review. Journal of addiction medicine. 2019 Jan 1;13(1):7-22.
21. Gong ZS, Guo QY, Zhang J, Gong S, Liu F, Xu L. Uncertainty Evaluation for Determination of Sibutramine in Health Food by HPLC-MS/MS. Natural Product Communications. 2024 Oct;19(10):1934578X241292937.
22. Nguyen TN, Nguyen HK, Nguyen TN, Duong TN, Pham TT, Nguyen TK. Validation of sibutramine and phenolphthalein determination by HPLC-PDA in natural dietary supplements for body-weight reduction. Food Research. 2024 Dec;8(6):162-9.
23. Suparmi S, Yuliyanti S, Karyadini HW, Syamsudin AM, Gau EK. Toxicity of sibutramine hydrochloride-adulterated weight loss supplements in rats based on biochemical and organ weight parameters. Journal of Pharmacy & Pharmacognosy Research. 2024;12(2):363-70.
24. Sembiring BM, Fahdi F, Tanjung DA, Faisal B. Investigation of herbal medicine identified sibutramine hcl through analysis of thin layer chromatography and uv-vis spectrophotometry methods. Rasayan journal of chemistry. 2024 oct 1;17(4).
25. Chorilli M, Bonfilio R, da Silva Chicarelli R, Salgado HR. Development and validation of an analytical method by RP-HPLC for quantification of sibutramine hydrochloride in pharmaceutical capsules. Analytical Methods. 2011;3(4):985-90.
26. Kilicarslan G, Imamoglu E, Kucuk A, Özdemir A. UV spectrophotometric, derivative spectrophotometric and RP-HPLC-DAD determination of sibutramine. Reviews in Analytical Chemistry. 2010 Dec;29(3-4):169-96.
27. Imran MD, PATHADE P. Development and validation of stability indicating uv spectrophotometric method for the estimation of sibutramine hydrochloride monohydrate in bulk and capsule dosage form. Int J Pharm Pharm Sci. 2011;3(4):53-6.
28. Hwang YY, Shin DC, Nam YS, Cho BK. Characterization, stability, and pharmacokinetics of sibutramine/β-cyclodextrin inclusion complex. Journal of Industrial and Engineering Chemistry. 2012 Jul 25;18(4):1412-7.
29. Ayad M, Abdellatef H, Hosny M, Kabil N. Utility of molybdophosphoric acid in qualitative and quantitative analysis of Sibutramine HCl, Sumatribtan succinate and Lomefloxacine HCl. International Journal of Pharmaceutical and Biomedical Research. 2012;3(2):121-26.
30. Singh AK, Garca PL, Gomes FP, Yano HM, Auricchio MT, Kedor-Hackmann ER, Santoro MI. Development and validation of sensitive methods for determination of sibutramine hydrochloride monohydrate and direct enantiomeric separation on a protein-based chiral stationary phase. Journal of AOAC International. 2008 May 1;91(3):572-9.
31. Maghfirah R, Farida M, Purnama A, Afifi MR, MZ K, Rahmawati E. Quantitative Analysis of Sibutramine Hydrochloride in Slimming Herbal Medicines in Banda Aceh Using UV-Vis Spectrophotometry. BIOLOGICA SAMUDRA. 2025 Jun 30;7(1):54-62.
32. Simaremare ES, Susilowati RA, Astuti YD, Hermawan R, Gunawan E, Pratiwi RD. Analysis of acetaminophen, mefenamic acid, sibutramine hydrochloride, and sildenafil citrate. Journal of Applied Pharmaceutical Science. 2018 Nov 30;8(11):048-56.
33. Yenare SS, Tare M, Navasupe PS, Bangar AB, Bole SS. UV spectroscopic method for estimation of topiramate bulk and pharmaceutical dosage forms.
34. Pinto EC, Dolzan MD, Cabral LM, Armstrong DW, Sousa VP. Topiramate: a review of analytical approaches for the drug substance, its impurities and pharmaceutical formulations. Journal of chromatographic science. 2016 Feb 1;54(2):280-90.
35. Ibrahim FA, El-Yazbi AF, Wagih MM, Barary MA. Sensitive inexpensive spectrophotometric and spectrofluorimetric analysis of ezogabine, levetiracetam and topiramate in tablet formulations using Hantzsch condensation reaction. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2017 Sep 5;184:47-60.
36. El-Yazbi AF, Wagih MM, Ibrahim F, Barary MA. Spectrofluorimetric determination of topiramate and levetiracetam as single components in tablet formulations and in human plasma and simultaneous fourth derivative synchronous fluorescence determination of their co-adminstered mixture in human plasma. Journal of fluorescence. 2016 Jul;26(4):1225-38.
37. Khan FI, Rehman MT, Sameena F, Hussain T, AlAjmi MF, Lai D, Khan MK. Investigating the binding mechanism of topiramate with bovine serum albumin using spectroscopic and computational methods. Journal of Molecular Recognition. 2022 Jul;35(7):e2958.
38. Mafi Z, Sohrabi MR, Davallo M. Fast and facile UV-spectrophotometry-assisted chemometrics methods for the simultaneous determination of phenytoin and phenobarbital as antiepileptic drugs in combined dosage form and urine sample. Iran. J. Chem. Chem. Eng.(IJCCE) Research Article Vol. 2024 Sep 3;43(9).
39. Sunitha PG, Deattu N, Dhanalakshmi R, Gangatharan A, Kavi BA, Shankar GM, Sathya M. Colorimetric methods for the estimation of topiramate in tablets. J. Drug Deliv. Ther. 2016;6:11-3.
40. Nejdar P, Salunkhe S, Pishawikar S, Uchgaon K. Development of Validated RP-HPLC Method for Determination of Sibutramine Applying QbD Approach.
41. Nguyen TN, Nguyen HK, Nguyen TN, Duong TN, Pham TT, Nguyen TK. Validation of sibutramine and phenolphthalein determination by HPLC-PDA in natural dietary supplements for body-weight reduction. Food Research. 2024 Dec;8(6):162-9.
42. Kozhuharov VR, Chakarov D, Ivanova S, Ivanov K. Development and validation of a high-performance thin-layer chromatography method for detection of sibutramine in dietary supplements. Folia Medica. 2024 Apr 30;66(2):255-63.
43. Gürbüzer A, Ulusoy HI, Narin İ. Sensitive Detection of Sibutramine and Fluoxetine Adulteration in Herbal Slimming Products by Means of Using Magnetic Solid Phase Extraction Combined with HPLC–DAD. Food Analytical Methods. 2025 Mar;18(3):459-70.
44. Ping T, Zheng M, Zhang P, Yan T, Miao X, Wang K, Lian K. Determination of 12 anti-obesity drugs in human plasma by a 96-well protein precipitation plate using HPLC-MS. RSC advances. 2022;12(40):26016-22.
45. Simaremare ES, Susilowati RA, Astuti YD, Hermawan R, Gunawan E, Pratiwi RD. Analysis of acetaminophen, mefenamic acid, sibutramine hydrochloride, and sildenafil citrate. Journal of Applied Pharmaceutical Science. 2018 Nov 30;8(11):048-56.
46. Kimani M, Lanzarotta A, Batson J. Analysis of unlabeled sibutramine in dietary supplements using surface-enhanced Raman spectroscopy (SERS) with handheld devices.
47. Qin L, Wang X, Lu D. Quantitative determination and validation of topiramate and its tablet formulation by 1 H-NMR spectroscopy. Analytical Methods. 2019;11(5):661-8.
48. Salzmann L, Spescha T, Singh N, Kobel A, Fischer V, Schierscher T, Bauland F, Geistanger A, Risch L, Geletneky C, Seger C. An isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)-based candidate reference measurement procedure for the quantification of topiramate in human serum and plasma. Clinical Chemistry and Laboratory Medicine (CCLM). 2023 Oct 26;61(11):1942-54.
49. Kuczyńska j, zakrzewska-sito a, bochyńska a, sienkiewicz-jarosz h, dermanowski m, mierzejewski p. Development of method for determining topiramate in various biological matrices (plasma, saliva, hair) and its application in clinical practice. Acta Poloniae Pharmaceutica. 2024 Jan 1;81(1).
50. Thumbar H, Dhalani J, Patel H, Dhaduk B. In Situ Identification and Quantification of Genotoxic Sulfonyl Chloride Impurity in Topiramate via LC-MS/MS. MethodsX. 2025 Jun 14:103441.
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Aug 20, 2025
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Sweety R. Patel, Dr. Neha Tiwari, & Dr. Pragnesh Patani. (2025). A REVIEW ON ANALYTICAL METHOD FOR ESTIMATION OF SIBUTRAMINE HYDROCHLORIDE AND TOPIRAMATE IN SYNTHETIC MIXTURE. Journal of Population Therapeutics and Clinical Pharmacology, 32(7), 1845-1851. https://doi.org/10.53555/tt83d327
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Author Biographies
Sweety R. Patel
Khyati College of Pharmacy, Gujarat Technological University, Ahmedabad, Gujarat, India.
Dr. Neha Tiwari
Department of Pharmaceutical Chemistry, Khyati College of Pharmacy, Gujarat Technological University, Ahmedabad, Gujarat, India.
Dr. Pragnesh Patani
Department of Pharmacology, Khyati College of Pharmacy, Gujarat Technological University, Ahmedabad, Gujarat, India.
How to Cite
Sweety R. Patel, Dr. Neha Tiwari, & Dr. Pragnesh Patani. (2025). A REVIEW ON ANALYTICAL METHOD FOR ESTIMATION OF SIBUTRAMINE HYDROCHLORIDE AND TOPIRAMATE IN SYNTHETIC MIXTURE. Journal of Population Therapeutics and Clinical Pharmacology, 32(7), 1845-1851. https://doi.org/10.53555/tt83d327