ANTI-INFLAMMATORY IMPACT: MULTIPLE USE OF TOPICAL THERAPEUTIC AGENTS IN WOUND REPAIR
Main Article Content
Keywords
wound, anti-inflammatory, zinc oxide, neutrophil, lymphocyte
Abstract
Objective: To assess the multiple therapeutic agents and an anti-inflammatory role in wound management
Place and duration of the study: The study was done at Al-Tibri Medical College and Hospital, from Jan 2024 to March 2025.
Methodology: The experimental study was done with 24 numbers of male albino rats, the rats were randomly selected between the weights of 150-250gms. The animal were divided into three groups, control treated with normal saline, another treated with eusol and one is treated with zinc oxide for 14 days. The subcutaneous wound was created on the dorsal surface of the rats of 2x2cm2 after anesthetized with ethanol. The sample were taken from the margins of the wound at day 3, 7 & 14. The tissue were processed and stained with Haematoxylin & Eosin for microscopy. At x400 the inflammatory cells were calculated through micrometry. The data were evaluated through SPSS, mean±SD of cells and compare after applied one way ANOVA followed by post hoc tuckey’s test. The level of significance was set at P=<0.05.
Results: Total Mean of neutrophil count found to be at low in group treated with zinc oxide, P=<0.001 as compared with others. Mean lymphocyte count was increased in zinc oxide treated group on day 3and 7, P=<0.001 that stimulate the granulation process.
Conclusion: The significant rapid wound repair was found in topical applied zinc oxide group, and decreased cell count evidence the anti-inflammatory impact at day 3 & 7 as compared to others. Zinc oxide is safer, cheaper and easy available compound with potent role in wound management.
References
2. Leise BS. Topical wound medications. Veterinary Clinics: Equine Practice. 2018;1;34(3):485-98.
3. Söderberg TA. Trace elements in normal and impaired wound healing. In Wound Healing and Skin Physiology 1995 (pp. 183-199). Berlin, Heidelberg: Springer Berlin Heidelberg.
4. Andrews M, Gallagher-Allred C. The role of zinc in wound healing. Advances in Skin & Wound Care. 1999;1;12(3):137.
5. Granum B. Opinion of the Scientific Committee on Consumer safety (SCCS)–Final opinion on water-soluble zinc salts used in oral hygiene products. Regulatory Toxicology and Pharmacology. 2018;1;99:249-50.
6. Kim KB, Kim YW, Lim SK, Roh TH, Bang DY, Choi SM, Lim DS, Kim YJ, Baek SH, Kim MK, Seo HS. Risk assessment of zinc oxide, a cosmetic ingredient used as a UV filter of sunscreens. Journal of Toxicology and Environmental Health, Part B. 2017;3;20(3):155-82.
7. Pandey PK, Koushariya M, Shukla S, Das S. Outcomes of superoxide solution dressings in surgical wounds: a randomized case control trial. Int J Biol Med Res. 2011;2(4):965-8.
8. Aggarwal R, Goel N, Chaudhary U, Kumar V, Ranjan KP. Evaluation of microbiocidal activity of superoxidized water on hospital isolates. Indian Journal of Pathology and Microbiology. 2010;1;53(4):757-9.
9. Eftekharizadeh F, Dehnavieh R, Hekmat SN, Mehrolhassani MH. Health technology assessment on super oxidized water for treatment of chronic wounds. Medical journal of the Islamic Republic of Iran. 2016;7;30:384.
10. Ågren MS, Phothong N, Burian EA, Mogensen M, Hædersdal M, Jorgensen LN. Topical zinc oxide assessed in two human wound-healing models. Acta dermato-venereologica. 2021;26;101(5):712.
11. Cangul IT, Gul NY, Topal A, Yilmaz R. Evaluation of the effects of topical tripeptide‐copper complex and zinc oxide on open‐wound healing in rabbits. Veterinary dermatology. 2006;17(6):417-23.
12. Abdullah BJ, Atasoy N, Omer AK. Evaluate the effects of platelet rich plasma (PRP) and zinc oxide ointment on skin wound healing. Annals of Medicine and Surgery. 2019;1;37:30-7.
13. Hashemi SS, Pakdin A, Mohammadi A, Keshavarzi A, Mortazavi M, Sanati P. Study the effect of calendula officinalis extract loaded on zinc oxide nanoparticle cream in burn wound healing. ACS Applied Materials & Interfaces. 2023;12;15(51):59269-79.
14. Okeniyi JA, Olubanjo OO, Ogunlesi TA, Oyelami OA. Comparison of healing of incised abscess wounds with honey and EUSOL dressing. Journal of Alternative & Complementary Medicine. 2005;1;11(3):511-3.
15. Ullah F, Rashid I, Ihsan A, Anwar A, Ullah H, Khan A. Efficacy of EUSOL, Neem, and TCDO in Full-Thickness Wound Healing: Histological and Hematological Assessment in Rabbits. Journal of Animal and Plant Research. 2025;29;2(2):51-66.
16. Shah AB, Maharjan R, Shrestha BP, Chaudhary P. A randomized controlled trial comparing EUSOL versus antibiotic loaded collagen granules as dressing agents in the management of traumatic infected wounds. International Journal of Orthopaedics. 2017;3(2):157-62.
Article Sidebar
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
All articles published in JPTCP are licensed under Copyright Creative Commons Attribution-NonCommercial 4.0 International License.
Mehr Fatima
Assistant Professor, Department of Pharmacology, Bahria University Health Sciences Campus, Karachi-Pakistan
Muhammed Baqir Soomro
Professor, Department of Anatomy, Jinnah Medical and Dental College Bahadurabad Karachi-Pakistan
Mariam Bashir Pavgi
Assistant Professor, Department of Anatomy, Sir Syed College of Medical Sciences for Girls Karachi-Pakistan
Nadia Naeem
Assistant Professor, Department of Pharmacology, Jinnah Sindh Medical University Karachi-Pakistan
Sana Kashif
Assistant Professor, Department of Anatomy, Chandka Medical College/SMBBMU Larkana - Pakistan
Sarah Sughra
Assistant Professor, Department of Anatomy, Sir Syed College of Medical Sciences for Girls Karachi-Pakistan
Sonia Khan
Associate Professor, Department of Pharmacology, Al-Tibri Medical College, Karachi, Pakistan