“HEMOGLOBINOPATHIES IN CHILDREN OF TRIBAL REGION OF MADHYA PRADESH: CORRELATION OF HEMATOLOGICAL INDICES AND HPLC”
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Keywords
Hemoglobinopathies, Pediatrics, Hematological Indices, HPLC, Thalassemia, Hemoglobin Variants, Red Cell Indices
Abstract
Background: Hemoglobinopathies are among the most common inherited disorders worldwide, particularly prevalent in paediatric populations in certain geographical regions. Early diagnosis using haematological indices and confirmatory techniques such as High Performance Liquid Chromatography (HPLC) is essential for effective disease management and genetic counselling.
Objective: To evaluate the spectrum of hemoglobinopathies in the paediatric age group using hematological indices and to correlate these findings with HPLC results.
Methods: This cross-sectional study was conducted on paediatric patients (aged 0–13 years) suspected of having hemoglobinopathies. Complete blood counts and red cell indices (Hb, RBC count, MCV, MCH, MCHC, RDW) were recorded using automated hematology analysers. Based on suggestive hematological parameters, samples were further analysed using HPLC for definitive diagnosis. Data were statistically analysed to assess the correlation between hematological indices and HPLC findings.
Results: Out of the total samples analysed, a significant proportion showed abnormal hematological indices suggestive of thalassemia traits or other hemoglobin variants. HPLC confirmed diagnosis including β-thalassemia trait, HbE trait, HbS trait, and compound heterozygous states. A strong correlation was found between reduced MCV/MCH values and β-thalassemia trait, while normal indices were seen in some hemoglobin variants. HPLC proved crucial for differentiating between similar phenotypes on hematological grounds.
Conclusion: Hematological indices serve as a useful screening tool for suspected hemoglobinopathies in children, but HPLC remains indispensable for accurate diagnosis and characterization. Early identification is vital for appropriate clinical management and genetic counselling.
References
2.Galanello R. SCREENING AND DIAGNOSIS FOR HAEMOGLOBIN DISORDERS. In: Angastiniotis M, Eleftheriou A, Galanello Ret al., authors; Old J, editor. Prevention of Thalassaemias and Other Haemoglobin Disorders: Volume 1: Principles [Internet]. 2nd edition. Nicosia (Cyprus): Thalassaemia International Federation; 2013.
3.Khera R, Singh T, Khuana N, Gupta N, Dubey AP. HPLC in characterization of hemoglobin profile in thalassemia syndromes and hemoglobinopathies: a clinicohematological correlation. Indian J Hematol Blood Transfus. 2015 Mar;31(1):110-5.
4.da Fonseca SF, Amorim T, Purificação A, Gonçalves M, Boa-Sorte N. Hemoglobin A2 values in sickle cell disease patients quantified by high performance liquid chromatography and the influence of alpha thalassemia. Rev Bras Hematol Hemoter. 2015 Sep-Oct;37(5):296-301.
5.Cianciulli P. Iron chelation therapy in thalassemia syndromes. Mediterr J Hematol Infect Dis. 2009 Dec 29;1(1):e2009034.
6.Twum S, Fosu K, Felder RA, Sarpong KAN. Bridging the gaps in newborn screening programmes: Challenges and opportunities to detect haemoglobinopathies in Africa. Afr J Lab Med. 2023 Dec 14;12(1):2225.
7.Buch, Archana; Iqbal, Banyameen; Bordawekar, Rajiv1; Jain, Ambuj; Jariwala, Pratik; Rathod, Hetal2 Patterns of hemoglobinopathies diagnosed by high-performance liquid chromatography in and around Pune (Western Maharashtra, India, Journal of Medical Society 30(2):p 111-115, May–Aug 2016. | DOI: 10.4103/0972-4958.182921
8.Roshan B Colah 1,✉, Malay B Mukherjee 1, Snehal Martin 1, Kanjaksha Ghosh 1 Sickle cell disease in tribal populations in India. Indian J Med Res. 2015 May;141(5):509–515. doi: 10.4103/0971-5916.159492
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Dr. Madhubala Chauhan
Junior Resident, Department of Pathology, Shyam Shah Medical College and associated hospitals, Rewa (M.P.).
Dr.Santosh Singh
Associate Professor, Department of Pathology, Shyam Shah Medical College and associated hospitals, Rewa (M.P.).
Dr.Jagannath Jatav
Associate Professor, Department of Pathology, Shyam Shah Medical College and associated hospitals, Rewa (M.P.).
Dr. s.k. sutrakar
Professor, Department of Pathology, Shyam Shah Medical College and associated hospitals, Rewa (M.P.)