EXPLORING THE POTENTIAL OF BIOSURFACTANT-PRODUCING ENDOPHYTIC PSEUDOMONAS AERUGINOSA SF1R IN MEDICAL THERAPEUTICS
Main Article Content
Keywords
Biosurfactant, Antibacterial, antioxidant, Antibiofilm, Pseudomonas aeruginosa
Abstract
The emergence of antibiotic resistance and demand for eco-friendly therapeutic agents have intensified interest in microbial biosurfactants with biomedical applications. This study explores the potential of Pseudomonas aeruginosa SF1R, an endophytic strain isolated from Mangifera indica, as a biosurfactant producer with promising therapeutic relevance. SF1R isolate was screened and selected for biosurfactant production followed by medical applications including antibacterial, antibiofilm and antioxidant activity. SFIR strain produced crude biosurfactant product of approximately 1117 mg/L with functional activities like 60 mm oil displacement activity, 38% emulsification index and strong activity in terms of drop collapse test. Biosurfactant produced by SF1R isolate showed maximum antibacterial activity (41 ± 1.5 mm) against E.coli followed by S. typhae (35.5 ± 0.7 mm). The biosurfactant resulted in 57% inhibition of the biofilm produced by S. aureus, followed by 16% inhibition of the biofilm of E. coli. Likewise, in biofilm eradication, 55% was observed in S. aureus followed by E.coli (28%). However, antioxidant activity in the form RSA of biosurfactant was observed as 60%.
References
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29. Sharma D, Singh Saharan B. Simultaneous production of biosurfactants and bacteriocins by probiotic Lactobacillus casei MRTL3. International Journal of microbiology. 2014;2014(1):698713.
30. Singh M, Kumar A, Singh R, Pandey KD. Endophytic bacteria: a new source of bioactive compounds. 3 Biotech. 2017 Oct;7:1-4.
31. Sotirova A, Spasova D, Vasileva-Tonkova E, Galabova D. Effects of rhamnolipid-biosurfactant on cell surface of Pseudomonas aeruginosa. Microbiological Research. 2009 Jan 1;164(3):297-303.
32. Sun S, Wang Y, Zang T, Wei J, Wu H, Wei C, Qiu G, Li F. A biosurfactant-producing Pseudomonas aeruginosa S5 isolated from coking wastewater and its application for bioremediation of polycyclic aromatic hydrocarbons. Bioresource technology. 2019 Jun 1;281:421-8.
33. Van Bogaert IN, Saerens K, De Muynck C, Develter D, Soetaert W, Vandamme EJ. Microbial production and application of sophorolipids. Applied microbiology and biotechnology. 2007 Aug;76:23-34.
34. Vater J, Kablitz B, Wilde C, Franke P, Mehta N, Cameotra SS. Matrix-assisted laser desorption ionization-time of flight mass spectrometry of lipopeptide biosurfactants in whole cells and culture filtrates of Bacillus subtilis C-1 isolated from petroleum sludge. Applied and environmental microbiology. 2002 Dec;68(12):6210-9.
35. Wei YH, Lai HC, Chen SY, Yeh MS, Chang JS. Biosurfactant production by Serratia marcescens SS-1 and its isogenic strain SMΔR defective in SpnR, a quorum-sensing LuxR family protein. Biotechnology letters. 2004 May;26:799-802.
36. Wolfmeier H, Pletzer D, Mansour SC, Hancock RE. New perspectives in biofilm eradication. ACS infectious diseases. 2018 Feb 9;4(2):93-106.
37. Wu H, Yang H, You X, Li Y. Isolation and characterization of saponin-producing fungal endophytes from Aralia elata in Northeast China. International journal of molecular sciences. 2012 Nov 30;13(12):16255-66.
2. Abdel-Mawgoud AM, Hausmann R, Lépine F, Müller MM, Déziel E. Rhamnolipids: detection, analysis, biosynthesis, genetic regulation, and bioengineering of production. Biosurfactants: from genes to applications. 2011:13-55.
3. Alej CS, Humberto HS, María JF. Production of glycolipids with antimicrobial activity by Ustilago maydis FBD12 in submerged culture. Afr J Microbiol Res. 2011 Sep 9;5(17):2512-23.
4. Balan SS, Kumar CG, Jayalakshmi S. Pontifactin, a new lipopeptide biosurfactant produced by a marine Pontibacter korlensis strain SBK-47: Purification, characterization and its biological evaluation. Process Biochemistry. 2016 Dec 1;51(12):2198-207.
5. Burch AY, Browne PJ, Dunlap CA, Price NP, Lindow SE. Comparison of biosurfactant detection methods reveals hydrophobic surfactants and contact‐regulated production. Environmental microbiology. 2011 Oct;13(10):2681-91.
6. Coronel-León J, de Grau G, Grau-Campistany A, Farfan M, Rabanal F, Manresa A, Marqués AM. Biosurfactant production by AL 1.1, a Bacillus licheniformis strain isolated from Antarctica: production, chemical characterization and properties. Annals of microbiology. 2015 Dec;65:2065-78.
7. Das P, Yang XP, Ma LZ. Analysis of biosurfactants from industrially viable Pseudomonas strain isolated from crude oil suggests how rhamnolipids congeners affect emulsification property and antimicrobial activity. Frontiers in microbiology. 2014 Dec 22;5:696.
8. Donio MB, Ronica SF, Viji VT, Velmurugan S, Jenifer JA, Michaelbabu M, Citarasu T. Isolation and characterization of halophilic Bacillus sp. BS3 able to produce pharmacologically important biosurfactants. Asian Pacific journal of tropical medicine. 2013 Nov 1;6(11):876-83.
9. Dusane DH, Pawar VS, Nancharaiah YV, Venugopalan VP, Kumar AR, Zinjarde SS. Anti-biofilm potential of a glycolipid surfactant produced by a tropical marine strain of Serratia marcescens. Biofouling. 2011 Jun 23;27(6):645-54.
10. Fooladi T, Moazami N, Abdeshahian P, Kadier A, Ghojavand H, Yusoff WM, Hamid AA. Characterization, production and optimization of lipopeptide biosurfactant by new strain Bacillus pumilus 2IR isolated from an Iranian oil field. Journal of Petroleum Science and Engineering. 2016 Sep 1;145:510-9.
11. Gaur VK, Sharma P, Gupta S, Varjani S, Srivastava JK, Wong JW, Ngo HH. Opportunities and challenges in omics approaches for biosurfactant production and feasibility of site remediation: strategies and advancements. Environmental Technology & Innovation. 2022 Feb 1;25:102132.
12. Ghasemi A, Moosavi-Nasab M, Setoodeh P, Mesbahi G, Yousefi G. Biosurfactant production by lactic acid bacterium Pediococcus dextrinicus SHU1593 grown on different carbon sources: strain screening followed by product characterization. Scientific reports. 2019 Mar 27;9(1):5287.
13. Goswami D, Handique PJ, Deka S. Rhamnolipid biosurfactant against Fusarium sacchari—the causal organism of pokkah boeng disease of sugarcane. Journal of Basic Microbiology. 2014 Jun;54(6):548-57.
14. Gudiña EJ, Rocha V, Teixeira JA, Rodrigues LR. Antimicrobial and antiadhesive properties of a biosurfactant isolated from Lactobacillus paracasei ssp. paracasei A20. Letters in applied microbiology. 2010 Apr 1;50(4):419-24.
15. Janek T, Łukaszewicz M, Krasowska A. Antiadhesive activity of the biosurfactant pseudofactin II secreted by the Arctic bacterium Pseudomonas fluorescens BD5. BMC microbiology. 2012 Dec;12:1-9.
16. Kitamoto D, Isoda H, Nakahara T. Functions and potential applications of glycolipid biosurfactants—from energy-saving materials to gene delivery carriers—. Journal of bioscience and bioengineering. 2002 Sep 1;94(3):187-201.
17. Lodewyckx C, Vangronsveld J, Porteous F, Moore ER, Taghavi S, Mezgeay M, der Lelie DV. Endophytic bacteria and their potential applications. Critical reviews in plant sciences. 2002 Nov 1;21(6):583-606.
18. Mahmoud YA, El-Halmouch YH, Nasr EE, Al-Sodany YM, El-Nogoumy BA, Ali SS. Exploring the potential of biosurfactants produced by fungi found in soil contaminated with petrochemical wastes. Scientific Reports. 2024 Oct 28;14(1):25733.
19. Mishra A, Singh SP, Mahfooz S, Bhattacharya A, Mishra N, Shirke PA, Nautiyal CS. Bacterial endophytes modulates the withanolide biosynthetic pathway and physiological performance in Withania somnifera under biotic stress. Microbiological Research. 2018 Jul 1;212:17-28.
20. Morita T, Fukuoka T, Imura T, Kitamoto D. Production of mannosylerythritol lipids and their application in cosmetics. Applied microbiology and biotechnology. 2013 Jun;97:4691-700.
21. Nair DN, Padmavathy SJ. Impact of endophytic microorganisms on plants, environment and humans. The Scientific World Journal. 2014;2014(1):250693.
22. Ohadi M, Forootanfar H, Rahimi HR, Jafari E, Shakibaie M, Eslaminejad T, Dehghannoudeh G. Antioxidant potential and wound healing activity of biosurfactant produced by Acinetobacter junii B6. Current pharmaceutical biotechnology. 2017 Sep 1;18(11):900-8.
23. Phulpoto IA, Qi Z, Qazi MA, Yu Z. Biosurfactants-based mixed polycyclic aromatic hydrocarbon degradation: from microbial community structure toward non-targeted metabolomic profile determination. Environment International. 2024 Feb 1;184:108448.
24. Phulpoto IA, Hu B, Wang Y, Ndayisenga F, Li J, Yu Z. Effect of natural microbiome and culturable biosurfactants-producing bacterial consortia of freshwater lake on petroleum-hydrocarbon degradation. Science of the Total Environment. 2021 Jan 10;751:141720.
25. Pisarska K, Pietr SJ. Bakterie endofityczne–ich pochodzenie i interakcje z roślinami. Post Mikrobiol. 2014;53:141-51.
26. Rau U, Nguyen LA, Roeper H, Koch H, Lang S. Fed-batch bioreactor production of mannosylerythritol lipids secreted by Pseudozyma aphidis. Applied microbiology and biotechnology. 2005 Sep;68:607-13.
27. Sana S, Datta S, Biswas D, Sengupta D. Assessment of synergistic antibacterial activity of combined biosurfactants revealed by bacterial cell envelop damage. Biochimica et Biophysica Acta (BBA)-Biomembranes. 2018 Feb 1;1860(2):579-85.
28. Shah MU, Sivapragasam M, Moniruzzaman M, Yusup SB. A comparison of recovery methods of rhamnolipids produced by Pseudomonas aeruginosa. Procedia engineering. 2016 Jan 1;148:494-500.
29. Sharma D, Singh Saharan B. Simultaneous production of biosurfactants and bacteriocins by probiotic Lactobacillus casei MRTL3. International Journal of microbiology. 2014;2014(1):698713.
30. Singh M, Kumar A, Singh R, Pandey KD. Endophytic bacteria: a new source of bioactive compounds. 3 Biotech. 2017 Oct;7:1-4.
31. Sotirova A, Spasova D, Vasileva-Tonkova E, Galabova D. Effects of rhamnolipid-biosurfactant on cell surface of Pseudomonas aeruginosa. Microbiological Research. 2009 Jan 1;164(3):297-303.
32. Sun S, Wang Y, Zang T, Wei J, Wu H, Wei C, Qiu G, Li F. A biosurfactant-producing Pseudomonas aeruginosa S5 isolated from coking wastewater and its application for bioremediation of polycyclic aromatic hydrocarbons. Bioresource technology. 2019 Jun 1;281:421-8.
33. Van Bogaert IN, Saerens K, De Muynck C, Develter D, Soetaert W, Vandamme EJ. Microbial production and application of sophorolipids. Applied microbiology and biotechnology. 2007 Aug;76:23-34.
34. Vater J, Kablitz B, Wilde C, Franke P, Mehta N, Cameotra SS. Matrix-assisted laser desorption ionization-time of flight mass spectrometry of lipopeptide biosurfactants in whole cells and culture filtrates of Bacillus subtilis C-1 isolated from petroleum sludge. Applied and environmental microbiology. 2002 Dec;68(12):6210-9.
35. Wei YH, Lai HC, Chen SY, Yeh MS, Chang JS. Biosurfactant production by Serratia marcescens SS-1 and its isogenic strain SMΔR defective in SpnR, a quorum-sensing LuxR family protein. Biotechnology letters. 2004 May;26:799-802.
36. Wolfmeier H, Pletzer D, Mansour SC, Hancock RE. New perspectives in biofilm eradication. ACS infectious diseases. 2018 Feb 9;4(2):93-106.
37. Wu H, Yang H, You X, Li Y. Isolation and characterization of saponin-producing fungal endophytes from Aralia elata in Northeast China. International journal of molecular sciences. 2012 Nov 30;13(12):16255-66.
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Sep 24, 2024
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Majid Ali Maitlo, Muneer Ahmed Qazi, & Abdul Hussain Shar. (2024). EXPLORING THE POTENTIAL OF BIOSURFACTANT-PRODUCING ENDOPHYTIC PSEUDOMONAS AERUGINOSA SF1R IN MEDICAL THERAPEUTICS. Journal of Population Therapeutics and Clinical Pharmacology, 31(9), 4378-4386. https://doi.org/10.53555/herncx95
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Author Biographies
Majid Ali Maitlo
Institute of microbiology, Shah Abdul Latif University, Khairpur, 66020
Muneer Ahmed Qazi
Institute of microbiology, Shah Abdul Latif University, Khairpur, 66020
Abdul Hussain Shar
Institute of microbiology, Shah Abdul Latif University, Khairpur, 66020
How to Cite
Majid Ali Maitlo, Muneer Ahmed Qazi, & Abdul Hussain Shar. (2024). EXPLORING THE POTENTIAL OF BIOSURFACTANT-PRODUCING ENDOPHYTIC PSEUDOMONAS AERUGINOSA SF1R IN MEDICAL THERAPEUTICS. Journal of Population Therapeutics and Clinical Pharmacology, 31(9), 4378-4386. https://doi.org/10.53555/herncx95