OPTIMIZATION OF ULTRASOUND-ASSISTED EXTRACTION AND PHYSICOCHEMICAL CHARACTERIZATION OF PEONY SEED OIL FOR FUNCTIONAL FOOD APPLICATIONS
Main Article Content
Keywords
Peony Seed Oil, Ultrasonic-Assisted Extraction (UAE), Alpha Linolenic Acid (ALA), Antioxidant Capacity, Response Surface Methodology (RSM)
Abstract
Peony seed oil (PSO) is a promising source of high-value edible oil, rich in polyunsaturated fatty acids and bioactive compounds. However, its industrial-scale extraction remains suboptimal. In this study, ultrasound-assisted extraction (UAE) was optimized using a single-factor experimental design followed by response surface methodology (RSM), employing Design-Expert® software for process modeling and regression analysis. The optimal extraction conditions 45 °C, 1.5 h, and a solvent-to-solid ratio of 8:1 yielded a maximum oil recovery of 22.98%. The physicochemical characterization revealed a favorable fatty acid composition dominated by α-linolenic acid (43.1%), linoleic acid (27.4%), and oleic acid (24.3%). Antioxidant activity was evaluated through DPPH, ABTS, and FRAPS assays, demonstrated over 85% radical scavenging capacity, indicating significant oxidative stability and health-promoting potential. Further analysis showed conjugated diene and triene values of 3.52 and 3.34, respectively, and high β-carotene content (2,267 mg/kg). Stability assessments under thermal stress revealed that PSO deteriorates with increasing temperature. Supplementation with tocopherol and peony seed polyphenol extracts improved oxidative stability, with tocopherol exhibiting the most pronounced protective effect. Collectively, these findings highlight ultrasound-assisted extraction as an efficient, green technology for producing nutritionally rich, bioactive oil from peony seeds. The study supports the use of PSO as a sustainable functional food ingredient and a potential alternative to conventional edible-oils.
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Nabila Jabbar
School of Chemistry and Chemical Engineering, North University of China, Taiyuan 030051, Shanxi, China
Li Gao
School of Chemistry and Chemical Engineering, North University of China, Taiyuan 030051, Shanxi, China
Haixia Ji
School of Chemistry and Chemical Engineering, North University of China, Taiyuan 030051, Shanxi, China
Zhengxuan He
School of Chemistry and Chemical Engineering, North University of China, Taiyuan 030051, Shanxi, China