Assessment of Phenolic and Flavonoid Contents and Free Radical Scavenging Activity of Ruellia tuberosa L. Stem Extracts
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Keywords
Anti-Oxidant Activity, DPPH free radical scavenging activity, Ruellia tuberosa, Total phenolic, phytoconstituents
Abstract
The present study was aimed to evaluate antioxidant activity of the Ruellia tuberosa (Acanthaceae) stem by utilizing in vitro models, specifically 2, 2-diphenyl-1-picrilhydrazyl (DPPH) radical scavenging activity and reducing power. Moreover, the total phenol and total flavonoid content were assessed using Folin-Ciocalteau reagent and colorimetric techniques with aluminum chloride respectively. Protein, carbohydrates, alkaloids, phenolic and flavonoid compounds were found in the preliminary phytochemical screening. The results demonstrated that the extract from R. tuberosa stem demonstrated DPPH radical scavenging action, when compared with standard ascorbic acid. The total phenolic and total flavonoid contents were found higher concentration in methanol extract than ethyl acetate extracts. It has been revealed that the Ruellia tuberosa stem displays antioxidant activity. The additional research could be done to figure out the active principle producing this effect.
References
2.Pham-Huy LA, He H, Pham-Huy C. Free radicals, antioxidants in disease and health. Int J Biomed Sci. 2008 Jun;4(2):89-96. PMID: 23675073; PMCID: PMC3614697.
3.Young IS, Woodside JV. Antioxidants in health and disease. J Clin Pathol. 2001 Mar;54(3):176-86. doi: 10.1136/jcp.54.3.176. PMID: 11253127; PMCID: PMC1731363
4.Javanraedi J, Stushnoff C, Locke E, Vivanco JM. Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chem.2003; 83, 547–550.
5.Wanasundara, P. K. J. P. D., & Shahidi, F. Optimigation of hexametaphosphate-assisted extraction of flaxseed proteins using response surface methodology. Journal of Food Science, 1996; 61, 604–607.
6.Zhang D, Yasuda T, Yu Y, Zheng P, Kawabata T, Ma Y, et al. Ginseng extract scavenges hydroxyl radical and protects unsaturated fatty acids from decomposition caused by iron mediated lipid peroxidation. Free Radical Biology and Medicine, 1996; 20, 145–150.
7.Chothani DL, Patel MB, Vaghasiya HU, Mishra SH. Review on Ruellia tuberosa (Cracker plant). Phcog J. 2010. Vol 2 (12)506–512.
8.Khare CP. Indian Medicinal Plants: An Illustrated Dictionary, Springer. 2007; p.561.
9.The wealth Of India, A Dictionary Of Indian, Raw material and Industrial product, Publication and Information Directorate, Council of Scientific and Industrial research, New Delhi: , 1972; 90.
10.Chothani DL, Mishra SH. In vitro anti-oxidant activity of Ruellia tuberosa root extracts. Free Radicals and Antioxidants. 2012 Oct 1;2(4):38-44.
11.Suseela L and Prema S. Pharmacognostic study on Ruellia tuberosa. Journal of Medicinal and aromatic Plant sciences. 2007; 29: 117–122.
12. Graham JG. et al.: Review; Plants used against cancer – an extension of the work of Jonathan Hartwell Journal of Ethnopharmacology. 2000; 73: 347–377
13. Kritikar KR and Basu BD. Indian medicinal plants. 1935; Vol. iii: 1866–67.
14.Chiu NY, Chang KH. The illustrated medicinal plants of Taiwan. Mingtong Medical J. 1995; 226: 1.
15.Kokate CK. Practical Pharmacognosy, 4th edition, Vallabh Prakashan, New Delhi, 2005, p. 107-111.
16.Singleton VL and Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic.1965; 16:144- 158.
17.C. N. Pandey, Medicinal plants of Gujarat, Gujarat Ecological Education and Research Foundation, Gujarat (India), 2005, p. 387
18.Chia-Chi Chang, Ming-Hua Yang, Hwei-Mei Wen, Jiing-chuan Chern, Estimation of Total Flavonoid content in propolis by two complementary colorimetric methods, Journal of food and drug analysis. 2002; 10(3):178-182.
19.Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity Songklanakarin J. Sci. Technol., 2004, 26(2): 211-219.
20.Shimada, K; Fujikawa, K; Nakamura, T; “Antioxidative properties of xanthan on the auto oxidation of soyabean oil in cyclodextrin emulsion”, J. Agric. Food. Chem. 1992; 40: pp 945-948.
21.Oyaizu, M; “Studies on product of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine”, Japanese Journal of Nutrition. 1986; 44, pp 307-315.
22.Zhi-hong Wang, Cheng-chin Hsu, Mei-chin Yin. Antioxidative characteristics of aqueous and ethanol extracts of glossy privet fruit. Food Chemistry.2009; 112: 914–918.
23.Shahidi F & Wanasundara PK, JPD. Phenolic antioxidants. Critical Reviews in Food Science and Nutrition, 1992; 32: 67–103.
24.Singleton VL, Rossi JA Jr. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture. 1965;16(3):144–158. doi: 10.5344/ajev.1965.16.3.144.
25.Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology. 1995;28(1):25–30. doi:10.1016/S0023-6438(95)80008-5.
26.Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958;181(4617):1199–1200. doi: 10.1038/1811199a0.
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