SEAWEED (SARGASSUM MUTICUM) MEDIATED GREEN SYNTHESIS OF AG2O NANOPARTICLES AND THEIR ANTIBACTERIAL PROPERTIES.
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Keywords
Ag₂O nanoparticles, Green synthesis, Antibacterial activity, Hemocompatibility, Antioxidant potential
Abstract
Introduction : Sargassum muticum is an invasive brown seaweed rich in bioactive metabolites with antimicrobial potential. Its abundance along coastal regions makes it a sustainable resource for green nanotechnology. Silver oxide (Ag,O) nanoparticles are well recognized for their antibacterial activity, and seaweed-mediated synthesis provides an eco-friendly alternative to conventional chemical methods. This study explores the use of Sargassum muticum extract for the green synthesis of Ag,O nanoparticles and evaluates their antibacterial efficacy.
Aim : To establish a green synthesis route for Ag₂O nanoparticles using Sargassum muticum extract and to evaluate their antibacterial, hemocompatibility, and antioxidant properties.
Materials and methods : Ag₂O nanoparticles were synthesized by reacting silver nitrate with S. muticum aqueous extract, followed by purification. Characterization was performed using UV–Vis spectroscopy, XRD, FESEM, and EDAX. Antibacterial activity was tested against Streptococcus mutans and Escherichia coli using disc diffusion assays. Hemocompatibility was assessed via hemolysis testing, and antioxidant activity was evaluated using the DPPH radical scavenging assay.
Results : The synthesized Ag₂O nanoparticles exhibited a distinct UV–Vis absorption band at 420–441 nm, confirming nanoparticle formation. FESEM revealed spherical particles ranging from 43–79 nm, while EDAX confirmed silver and oxygen composition. Antibacterial assays showed significant inhibition zones against both Gram‑positive and Gram‑negative strains. Hemolysis values (1.8–2.9%) remained below the 5% threshold, confirming blood safety. Antioxidant activity reached 75.8% compared to 89.2% for ascorbic acid.
Conclusion : The green synthesis of Ag₂O nanoparticles using Sargassum muticum extract yielded crystalline, biocompatible nanomaterials with potent antibacterial and antioxidant activity. These findings suggest that eco‑friendly Ag₂O nanoparticles are promising candidates for oral healthcare and systemic infection control, providing a sustainable alternative to conventional antimicrobial strategies.
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Vaishali Babu
Department of prosthodontics,Saveetha Dental College and Hospitals,Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai-600 077, Tamil Nadu, India.
L. Keerthi Sasanka
Senior Lecturer, Department of prosthodontics,Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai-600 077, Tamil Nadu, India.
Sathish Kumar Ramachandran
Professor, Department of Prosthodontics and Materials Science,Saveetha Dental College and hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai-600077, Tamil Nadu, India.
