BIOSYNTHESIS, CHARACTERISATION AND ANTIMICROBIAL ACTIVITY OF HIBISCUS AND MARIGOLD MEDIATED AGNPS
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Keywords
Silver Nanoparticles, Anti Microbial Activity, Green Technique.
Abstract
Aim: To analyze the biosynthesis, characterisation and anti-microbial activity of hibiscus and marigold mediated AgNPs.
Introduction: Pharmaceutical corporations are substantially investing in the development of natural medicines derived from plants as a result of the rise in the usage of traditional medicinal herbs in recent years. There are numerous potential actions and fields for nanoparticles. Nanoparticle chemical synthesis is no longer preferable to plant-based synthesis. Silver nanoparticles (AgNPs) are produced in the current work using a green technique and floral extract.
Materials and method: Mueller Hinton Agar was utilized for this activity to determine the zone of inhibition. Mueller hinton agar was prepared and sterilized for 15 minutes at 121oC. Media poured into the sterilized plates and let it stable for solidification. The wells were cut using a 9mm sterile polystyrene tip and the test organisms were swabbed. The nanoparticles with different concentrations (25µL, 50 µL ,100 µL )were loaded and in the fourth well standard antibiotic amoxyrite was loaded
Result: When silver nanoparticles were created using hibiscus and marigold extract, the anti-microbial properties of the nanoparticles were biosynthesized in a stunning way.In the MIC test, hibiscus rosa-sinensis extract of AgNps demonstrated good antibacterial effectiveness.
Conclusion: AgNPs may therefore be a good alternative to develop as an antibacterial agent against the diverse strains of bacteria that are drug-resistant.
References
2. Alam, M.K. et al. (2024) ‘Salivary Biomarkers and Temporomandibular Disorders: A Systematic Review conducted according to PRISMA guidelines and the Cochrane Handbook for Systematic Reviews of Interventions’, Journal of oral rehabilitation, 51(2), pp. 416–426.
3. Ameri, A., Ekhtelat, M. and Shamsaei, S. (2020) ‘Microbial indices of industrial and traditional medicinal herbs in Ahvaz, Iran’, Foods and Raw Materials, pp. 134–140. Available at: https://doi.org/10.21603/2308-4057-2020-1-134-139.
4. Anwar Ibrahim, D. ’a and Noman Albadani, R. (2014) ‘Evaluation of the Potential Nephroprotective and Antimicrobial Effect of Camellia sinensis Leaves versus Hibiscus sabdariffa (In Vivo and In Vitro Studies)’, Advances in pharmacological sciences, 2014, p. 389834.
5. Ashwlayan, V.D. et al. (2018) ‘Therapeutic Potential of Calendula officinalis’, Pharmacy & Pharmacology International Journal. Available at: https://doi.org/10.15406/ppij.2018.06.00171.
6. Gopinath, D. et al. (2021) ‘Compositional profile of mucosal bacteriome of smokers and smokeless tobacco users’, Clinical oral investigations [Preprint]. Available at: https://doi.org/10.1007/s00784-021-04137-7.
7. Grumezescu, A. (2016) Nanobiomaterials in Antimicrobial Therapy: Applications of Nanobiomaterials. William Andrew.
8. Guzman, M., Dille, J. and Godet, S. (2012) ‘Synthesis and antibacterial activity of silver nanoparticles against gram-positive and gram-negative bacteria’, Nanomedicine: nanotechnology, biology, and medicine, 8(1), pp. 37–45.
9. Haider, A.J., Haider, M.J. and Mehde, M.S. (2018) ‘A review on preparation of silver nano-particles’. Available at: https://doi.org/10.1063/1.5039273.
10. Joseph, B. and Anil, S. (2023) ‘Oral lesions in human monkeypox disease and their management-a scoping review’, Oral surgery, oral medicine, oral pathology and oral radiology, 135(4), pp. 510–517.
11. Khristi, V. and Patel, V.H. (2017) ‘THERAPEUTIC POTENTIAL OF HIBISCUS ROSA SINENSIS: A REVIEW’, International Journal of Nutrition and Dietetics, pp. 105–123. Available at: https://doi.org/10.17654/nd004020105.
12. Loo, Y.Y. et al. (2018) ‘Antimicrobial Activity of Green Synthesized Silver Nanoparticles Against Selected Gram-negative Foodborne Pathogens’, Frontiers in microbiology, 9, p. 1555.
13. Nayak, D. et al. (2015) ‘Biosynthesis, characterisation and antimicrobial activity of silver nanoparticles using Hibiscus rosa-sinensis petals extracts’, IET nanobiotechnology / IET, 9(5), pp. 288–293.
14. de Oliveira, M.S., Silva, S. and Da Costa, W.A. (2020) Essential Oils: Bioactive Compounds, New Perspectives and Applications. BoD – Books on Demand.
15. Pal, S., Tak, Y.K. and Song, J.M. (2007) ‘Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the Gram-negative bacterium Escherichia coli’, Applied and environmental microbiology, 73(6), pp. 1712–1720.
16. Patra, A. and Panja, A. (2013) Antimicrobial Activity in Ayurveda& Role of Herbs on Gram-ve Bacteria.
17. Peng, C.-H. et al. (2011) ‘Hibiscus sabdariffa polyphenolic extract inhibits hyperglycemia, hyperlipidemia, and glycation-oxidative stress while improving insulin resistance’, Journal of agricultural and food chemistry, 59(18), pp. 9901–9909.
18. Ronsivalle, V. et al. (2024) ‘Prevalence of temporomandibular disorders in juvenile idiopathic arthritis evaluated with diagnostic criteria for temporomandibular disorders: A systematic review with meta-analysis’, Journal of oral rehabilitation, 51(3), pp. 628–637.
19. Swarnavalli, G.C.J. et al. (2017) ‘Bio inspired synthesis of monodispersed silver nano particles using Sapindus emarginatus pericarp extract – Study of antibacterial efficacy’, Journal of Saudi Chemical Society, pp. 172–179. Available at: https://doi.org/10.1016/j.jscs.2015.03.004.
20. Uzunçıbuk, H. et al. (2024) ‘Prevalence of temporomandibular disorders in clear aligner patients using orthodontic intermaxillary elastics assessed with diagnostic criteria for temporomandibular disorders (DC/TMD) axis II evaluation: A cross-sectional study’, Journal of oral rehabilitation, 51(3), pp. 500–509.
21. Wang, C. et al. (2022) ‘Evaluation of the antimicrobial function of Ginkgo biloba exocarp extract against clinical bacteria and its effect on Staphylococcus haemolyticus by disrupting biofilms’, Journal of ethnopharmacology, 298, p. 115602.
22. Zaheer, Z. and Rafiuddin (2013) ‘Bio-conjugated silver nanoparticles: from Ocimum sanctum and role of cetyltrimethyl ammonium bromide’, Colloids and surfaces. B, Biointerfaces, 108, pp. 90–94.
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Priyanka. R
Undergraduate, bDepartment of Forensic Odontology, Saveetha Dental College and Hospitals,
Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai-600 077, Tamil Nadu, India.
Dr. Abirami Arthanari
Senior lecturer, Department of Forensic Odontology, Saveetha Dental College and Hospitals,
Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai-600 077, Tamil Nadu, India.
Dr. S. Rajeshkumar
Professor Department of Pharmacology Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai-600 077, Tamil Nadu, India.
